Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780134433769
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman
Publisher: PEARSON
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Chapter 8, Problem 13TYU
FOCUS ON ENERGY AND MATIER
Life is solar powered. Almost all the producers of the biosphere depend on energy from the sun to produce the organic molecules that supply the energy and carbon skeletons needed for life. In a short essay (100‒150 words), describe how the process of photosynthesis in the chloroplasts of plants transforms the energy of sunlight into the chemical energy of sugar molecules.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 8 Solutions
Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
Ch. 8.1 - How do the reactant molecules of photosynthesis...Ch. 8.1 - How did the use of an oxygen isotope help...Ch. 8.1 - WHAT IF? The Calvin cycle requires ATP and NADPH,...Ch. 8.2 - What color of light is least effective in driving...Ch. 8.2 - In the light reactions, what is the initial...Ch. 8.2 - Prob. 3CCCh. 8.3 - MAKE CONNECTIONS How are the large numbers of ATP...Ch. 8.3 - WHAT IF? Explain why a poison that inhibits an...Ch. 8.3 - Prob. 3CCCh. 8 - The light reactions of photosynthesis supply the...
Ch. 8 - Which of the following sequences correctly...Ch. 8 - How is photosynthesis similar in C4, plants and...Ch. 8 - Which of the following statements is a correct...Ch. 8 - Which of the following does not occur during the...Ch. 8 - In mechanism, photophosphorylation is most similar...Ch. 8 - Prob. 7TYUCh. 8 - To synthesize one glucose molecule, the Calvin...Ch. 8 - SCIENCE, TECHNOLOGY, AND SOCIETY Scientific...Ch. 8 - DRAW IT The following diagram represents an...Ch. 8 - Prob. 11TYUCh. 8 - Prob. 12TYUCh. 8 - FOCUS ON ENERGY AND MATIER Life is solar powered....Ch. 8 - SYNTHESIZE YOUR KNOWLEDGE Watermelon snow in...
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