Foundations in Microbiology
Foundations in Microbiology
9th Edition
ISBN: 9780073522609
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 6.8, Problem 31CYP
Summary Introduction

To analyse:

The differences between viruses and non-viral infectious particles like prions and viroids.

Introduction:

Viruses are extremely small infectious particles that are obligate parasites. They usually comprise of nucleic acid core surrounded by a protective coating of proteins. Prions and viroids are also infectious particles and cause diseases in many animals and plants, respectively.

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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin

Chapter 6 Solutions

Foundations in Microbiology

Ch. 6.2 - Prob. 7ELOCh. 6.2 - Summarize the different viral groups based on...Ch. 6.3 - Prob. 9ELOCh. 6.3 - Indicate the characteristics used in identifying...Ch. 6.3 - Prob. 5CYPCh. 6.3 - Describe the general structure of viruses.Ch. 6.3 - Prob. 7CYPCh. 6.3 - Prob. 8CYPCh. 6.3 - Prob. 9CYPCh. 6.3 - Prob. 10CYPCh. 6.3 - How are the poxviruses different from other animal...Ch. 6.3 - Prob. 12CYPCh. 6.3 - How are generic and common names used?Ch. 6.4 - Describe the virus-host relationship.Ch. 6.4 - Prob. 12ELOCh. 6.4 - Prob. 13ELOCh. 6.4 - Prob. 14ELOCh. 6.4 - Explain two ways in which animal viruses are...Ch. 6.4 - Prob. 16ELOCh. 6.4 - Write a narrative that describes the stages in the...Ch. 6.4 - Prob. 15CYPCh. 6.4 - Summarise the two major ways in which animal...Ch. 6.4 - Prob. 17CYPCh. 6.4 - Describe several cytopathic effects of viruses....Ch. 6.4 - Explain what it means for a virus to become...Ch. 6.4 - Prob. 20CYPCh. 6.5 - Prob. 17ELOCh. 6.5 - Explain what is meant by lysogeny, prophage,...Ch. 6.5 - Prob. 19ELOCh. 6.5 - In simple terms, what does the viral nucleic acid...Ch. 6.5 - What processes are involved in bacteriophage...Ch. 6.5 - Prob. 23CYPCh. 6.5 - Compare and contrast the main phases in the lytic...Ch. 6.5 - why is penetration so different between the two...Ch. 6.6 - Prob. 20ELOCh. 6.6 - Compare the methods and uses of cell culture, bird...Ch. 6.7 - Prob. 22ELOCh. 6.7 - Prob. 23ELOCh. 6.7 - Describe the three main techniques for cultivating...Ch. 6.7 - Prob. 27CYPCh. 6.7 - What are cell lines and monolayers, and how are...Ch. 6.7 - Prob. 29CYPCh. 6.8 - Prob. 24ELOCh. 6.8 - Prob. 25ELOCh. 6.8 - Prob. 30CYPCh. 6.8 - Prob. 31CYPCh. 6.L1 - Prob. 1MCQCh. 6.L1 - Prob. 2MCQCh. 6.L1 - The capsid is composed of protein subunits called...Ch. 6.L1 - Prob. 4MCQCh. 6.L1 - Prob. 5MCQCh. 6.L1 - Prob. 6MCQCh. 6.L1 - A prophage is a/an ____ stage in the cycle of...Ch. 6.L1 - Prob. 8MCQCh. 6.L1 - Prob. 9MCQCh. 6.L1 - Prob. 10MCQCh. 6.L1 - Prob. 11MCQCh. 6.L1 - Prob. 12MCQCh. 6.L1 - Prob. 13MCQCh. 6.L1 - Prob. 14MCQCh. 6.L1 - Prob. 15MCQCh. 6.L1 - Prob. 1CSRCh. 6.L1 - Prob. 2CSRCh. 6.L1 - Prob. 3CSRCh. 6.L1 - a. What characteristics of viruses could be used...Ch. 6.L1 - Prob. 2WCCh. 6.L1 - a. Since viruses lack metabolic enzymes, how can...Ch. 6.L1 - Prob. 4WCCh. 6.L2 - Prob. 1CTCh. 6.L2 - Prob. 2CTCh. 6.L2 - Prob. 3CTCh. 6.L2 - a. Given that DNA viruses can be carried in the...Ch. 6.L2 - Prob. 5CTCh. 6.L2 - Is there such a thing as a “good virus�?...Ch. 6.L2 - Prob. 7CTCh. 6.L2 - Prob. 8CTCh. 6.L2 - Prob. 9CTCh. 6.L2 - Label the parts of viruses in figures...Ch. 6.L2 - How would you describe the kind of capsid found on...
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What Is A Virus ? ; Author: Peekaboo Kidz;https://www.youtube.com/watch?v=YS7vsBgWszI;License: Standard YouTube License, CC-BY