LooseLeaf Benson's Microbiological Applications Laboratory Manual-Concise Version
LooseLeaf Benson's Microbiological Applications Laboratory Manual-Concise Version
14th Edition
ISBN: 9781259705236
Author: Alfred E Brown Ph.D., Heidi Smith
Publisher: McGraw-Hill Education
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Chapter 60, Problem 1SAQ
Summary Introduction

To describe: The happenings in the wells with agglutination.

Introduction: The process of formation of visible clumps due to the reaction takes place between an antigen and antibody is termed as agglutination. Agglutination occurs due to the reaction between the specific antibodies with the antigens present on the blood cell.

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The blood typing procedure is performed using the antisera possessing a high titer of anti-A and anti-B antibodies. The red blood cells possess two types of surface antigens, namely A and B antigens. The blood group was classified into four groups A, B, AB, and O on the basis of the presence of these antigens.

In the wells, the sample of blood is mixed with the antiserum containing the antibodies. The reaction between the antigen and antibody indicates the group of the blood. If agglutination occurs in the well containing anti-A antiserum, then the blood group is A. If agglutination occurs in the well containing anti-B antiserum, then the blood group is B. If agglutination occurs in the well containing both the anti-A and B antiserum, the blood group is AB. The absence of agglutination in both the wells containing anti-A and anti-B antiserum indicates the blood group of O.

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Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…
Question #5: Assume that two genes are identified that confer gametophytic facultative apomixis in soybean. The genes show independent assortment. Recessive alleles at both loci are required for the facultative apomixis. Facultative apomixis is triggered when the temperature at pollination is above 20 degrees C. At temperatures below 20 degrees C, all reproduction is sexual, independent of genotype. A facultative apomict male, capable of producing viable pollen, was crossed with a sexually reproducing female. Assuming the parents are completely inbred, what are the predicted phenotypic ratios (apomict: non-apomict) for the F1, F2, and DH (F1-derived) generations at each of the following temperatures*: a) 15°C? b) 25°C? *for full credit, show crosses and genotypes where appropriate. Remember to position the female first (left side) in the cross. Type your answer here:

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LooseLeaf Benson's Microbiological Applications Laboratory Manual-Concise Version

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