Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 6, Problem 6R
A pastry chef accidentally inoculated a cream pie with six S. aureus cells. If S. aureus has a generation time of 60 minutes, how many cells would be in the cream pie after 7 hours?
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If the generation time of Escherichia coli is 30 minutes, starting with 4 E. coli cells, how many cells can you obtain after 2 hours of growth?
A:
cells
You inoculated a culture with an initial cell count of 6.5x10^3 cells. The generation time for this organism is 25 minutes. You grew the culture for 10 hours.
a) How many generations occurred?b) How many cells will be present after the 10 hours?
You are doing a viable cell count for a culture of E. coli you are growing in a flask. The volume of media in the flask is 1L. You took an aliquot directly from the media and diluted it using trypan blue. Here are the numbers that you get:
squares counted: 4
total cells counted: 988
total viable cells counted: 960
THESE ARE THE QUESTIONS:
What is your cell viability?
What is the total number of viable cells in your flask?
Show all work.
Given:
Cell Viability = Viable Cells/Total Cells
Cells/mL = Total Viable Cells/Coners Counted x 10,000 x Dilution factor
Chapter 6 Solutions
Microbiology: An Introduction
Ch. 6 - Prob. 1RCh. 6 - Prob. 2RCh. 6 - Prob. 3RCh. 6 - Seven methods of measuring microbial growth were...Ch. 6 - By deep-freezing, bacteria can be stored without...Ch. 6 - A pastry chef accidentally inoculated a cream pie...Ch. 6 - Nitrogen and phosphorus added to beaches following...Ch. 6 - Differentiate complex and chemically defined...Ch. 6 - DRAW IT Draw the following growth curves for E....Ch. 6 - NAME IT A prokaryotic cell hitched a ride to Earth...
Ch. 6 - Two culture media were inoculated with four...Ch. 6 - Two culture media were inoculated with four...Ch. 6 - Use the following graph to answer questions 3 and...Ch. 6 - Use the following graph to answer questions 3 and...Ch. 6 - Assume you inoculated 100 facultatively anaerobic...Ch. 6 - The term trace elements refers to a. the elements...Ch. 6 - Which one of the following temperatures would most...Ch. 6 - Which of the following is not a characteristic of...Ch. 6 - Which of the following types of media would not be...Ch. 6 - An organism that has peroxidase and superoxide...Ch. 6 - E. coli was incubated with aeration in a nutrient...Ch. 6 - Clostridium and Streptococcus are both...Ch. 6 - Most laboratory media contain a fermentable...Ch. 6 - Flask A contains yeast cells in glucose-minimal...Ch. 6 - Clinical Applications and Evaluation 1. Assume...Ch. 6 - Heat lamps are commonly used to maintain foods at...Ch. 6 - The number of bacteria in saliva samples was...
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