Microbiology: An Introduction
Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Chapter 6, Problem 3A

Most laboratory media contain a fermentable carbohydrate and peptone because the majority of bacteria require carbon, nitrogen, and energy sources in these forms. How are these three needs met by glucose-minimal salts medium? (Hint: See Table 6.2.)

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Yeast cells are added to a 3.0 L batch bioreactor so that the initial cell concentration is [X]. = 1.3 g cells / L. The growth mediğim in the reactor, which is well-mixed so the cells have access to all nutrients, contains 150 g CELL DATA ribose (C5H10O5, MW 150), 75 g ammonia (NH3, MW 17), and 85 g oxygen (O2, MW 32). A-10. Determine the maximum concentration of cells, [X], that will form in the bioreactor when the limiting nutrient is consumed. Search Yx/ribose YX/02 YX/NH3 Specific growth rate on limiting nutrient, u Lag phase duration Deceleration phase duration 0.48 g/g 0.87 g/g 0.23 g/g 0.51 h 30 minutes Negligible
The nutrient broth is a basic media used for growing a broad variety of microorganisms in the laboratory. The nutrient broth consists mainly of 1.5g * L ^ - 1 extract, 3g * L ^ - 1 yeast extract and 5g * L ^ - 1 sodium chloride dissolved in distilled water. The nutrient agar is prepared by adding the agar at the desired amount into nutrient broth. Answer the following questions. marks) a) How would you prepare a 500 ml nutrient broth? b) How would you prepare 2% (w / v) nutrient agar for 1L? c) How would you prepare a 100 ml nutrient broth supplemented with 100mu * g / m * l ampicillin antibiotic? The stock concentration for ampicillin solution is 100mg / m * l .
You inoculate two test tubes with the same amount of identical growth medium and with the same number of identical yeast cells and grow these cells under identical conditions except for the presence or absence of oxygen. b) The cells of both cultures convert glucose to pyruvate via glycolysis, and then further metabolize pyruvate. Are the cells of both cultures able to obtain the same amount of ATP from glycolysis? Explain. Under anaerobic conditions, the carbon from pyruvate will ultimately be found in which molecule? Under aerobic conditions, the carbon from pyruvate will ultimately be found in which molecule?

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Microbiology: An Introduction

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