Microbiology: An Introduction
Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Chapter 6, Problem 1CAE

Clinical Applications and Evaluation

1. Assume that after washing your hands, you leave ten bacterial cells on a new bar of soap. You then decide to do a plate count of the soap after it was left in the soap dish for 24 hours. You dilute 1 g of the soap 1:106 and plate it on heterotrophic plate count agar. After 24 hours of incubation, there are 168 colonies. How many bacteria were on the soap? How did they get there?

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Worksheet 2. Draw it. Instruction: A bacterial culture was in log phase. At time x, an antibacterial compound was added to the culture. Draw the lines indicating addition of a bacterial compound and a bacteriostatic compound. Explain why the viable count does not immediately drop to zero at x? Time Log of number of cells
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Fit to 3. A group of students wants to study the effects of temperature on bacterial growth. To get bacteria, they leave petri dishes of nutrient agar open on a shelf. They then put the dishes in different places: an incubator (37 degrees C) a lab room (21 degrees C), a refrigerator (10 degrees C) and a freezer (0 degrees C). Bacterial growth is measured by estimating the percentage of each dish covered by the bacteria at the end of a three day growth period. a) What is the independent variable? b) What is the dependent variable? c) List two (or more) ways to improve this experimental design.

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Microbiology: An Introduction

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