EP MICROBIOLOGY:W/DISEASES BY..-MOD.ACC
5th Edition
ISBN: 9780134607894
Author: BAUMAN
Publisher: PEARSON CO
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Chapter 6, Problem 23CT
Viable plate counts are used to estimate
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You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL. List a series of dilutions and platings that you could carry out in order to determine the exact concentration of the culture. Note that you must plate four plates from a minimum of two dilution tubes. The volumes plated should be in the range of 0.1 mL – 1.0 mL. Duplicate volumes may not be plated from any one dilution tube. Each plating should aim for a count between 30 and 300 CFUs.
You can select any value from 30-300 for CFU and any volume from 0.1-1.0 to find out dilution scheme
Chapter 6 Solutions
EP MICROBIOLOGY:W/DISEASES BY..-MOD.ACC
Ch. 6 - Why should cardiac nurses and respiratory...Ch. 6 - Cavities Gone Wild Five-year-old Daniel appears to...Ch. 6 - Why do clinical laboratory scientists keep many...Ch. 6 - Boils in the Locker Room For several weeks,...Ch. 6 - Some students transfer some gunk from a 2-week-old...Ch. 6 - Which of the following can grow in a Petri plate...Ch. 6 - In the laboratory, a sterile inoculating loop is...Ch. 6 - Superoxide dismutase _____________. a. causes...Ch. 6 - The most reactive of the four toxic forms of...Ch. 6 - Microaerophiles that grow best with a high...
Ch. 6 - Prob. 6MCCh. 6 - Organisms that preferentially may thrive in icy...Ch. 6 - Prob. 8MCCh. 6 - Which of the following terms best describes an...Ch. 6 - In a defined medium, ______________. a. the exact...Ch. 6 - Which of the following is most useful in...Ch. 6 - Which of the following methods is best for...Ch. 6 - A Coulter counter is a(n) ________________. a....Ch. 6 - Lyophilization can be described as ___________. a....Ch. 6 - Quorum sensing is _____________. a. the ability to...Ch. 6 - Prob. 1FIBCh. 6 - Prob. 2FIBCh. 6 - Prob. 3FIBCh. 6 - Prob. 4FIBCh. 6 - Prob. 5FIBCh. 6 - Fill in the Blanks 6. Cells that shrink in...Ch. 6 - Fill in the Blanks 7. Obligate ________ exist in...Ch. 6 - Prob. 8FIBCh. 6 - Fill in the Blanks 9. Microbes that reduce N2 to...Ch. 6 - Fill in the Blanks 10. A student observes a...Ch. 6 - Fill in the Blanks 11. Chemolithotrophs acquire...Ch. 6 - Prob. 1VICh. 6 - Prob. 2VICh. 6 - High temperature affects the shape of particular...Ch. 6 - Support or refute the following statement:...Ch. 6 - Explain quorum sensing, and describe how it is...Ch. 6 - Why must media, vessels, and instruments be...Ch. 6 - Why is agar used in microbiology?Ch. 6 - What is the difference between complex media and...Ch. 6 - Draw and label the four distinct phases of a...Ch. 6 - If there are 47 cells in 1 l of sewage, how many...Ch. 6 - Prob. 9SACh. 6 - Prob. 10SACh. 6 - Explain the differences among photoautotrophs,...Ch. 6 - Contrast the media described in Tables 6.3 and 6.4...Ch. 6 - How does a chemostat maintain a constant...Ch. 6 - A scientist describes an organism as a...Ch. 6 - Pasteurization is a technique that uses...Ch. 6 - Two cultures of a facultative anaerobe are grown...Ch. 6 - Some organisms require riboflavin (vitamin B2) to...Ch. 6 - A scientist inoculates a bacterium into a complex...Ch. 6 - How can regions within biofilms differ in their...Ch. 6 - A scientific article describes a bacterium as an...Ch. 6 - Prob. 8CTCh. 6 - Prob. 9CTCh. 6 - Starting with 10 bacterial cells per milliliter in...Ch. 6 - Suppose you perform a serial dilution of 0.1-ml...Ch. 6 - How might the study of biofilms benefit humans?Ch. 6 - The filamentous bacterium Beggiatoa gets its...Ch. 6 - Given that Haemophilus ducreyi is a...Ch. 6 - Examine the graph in Figure 6.3. Note that the...Ch. 6 - Prob. 16CTCh. 6 - Using the terms in Figure 6.8a, describe the...Ch. 6 - Prob. 18CTCh. 6 - Prob. 19CTCh. 6 - Prob. 20CTCh. 6 - Prob. 21CTCh. 6 - Prob. 22CTCh. 6 - Viable plate counts are used to estimate...Ch. 6 - Using the following terms, fill in the following...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- A microbiologist inoculates a growth medium with 100 bacterial cells/ml. If the generation time of the species is 1 hour, and the microbes are in the exponential (log) phase of growth, how many hours require for the culture to surpass 10,000 cells/ml, if the culture is checked once each hour? O 1) 2 hours O 2) 7 hours O 3) 3 hours O 4) 10 hours O 5) 24 hoursarrow_forwardAssume an inoculum with a cell density of 108 cells per mL. The entire generation time takes 30 minutes. How many hours would it take to grow a culture to 108/mL if you started with a 10–2 dilution? helpful formula: g (generation time) = 0.301 (time)/ log x – log xoarrow_forwardAs shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number of colony forming units (CFU) per mL it contains. You do a plate count with these growth results (no. of colonies for each dilution): 1:10 too many to count; 1:100 too many to count; 1:1,000 312; 1:10,000 38; 1:100,000 no growth. The number of CFU per mL in the original culture was: A. 38 B. 380,000 C. 38,000 D. None of the other four answers (Correct answer not given) E. 10,000arrow_forward
- As shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number of colony forming units (CFU) per mL it contains. You do a plate count with these growth results (no. of colonies for each dilution): 1:10 too many to count; 1:100 too many to count; 1:1,000 174; 1:10,000 23; 1:100,000 no growth. The number of CFU per mL in the original culture was: 1 ml 1 ml Original inoculum Dilutions 9 ml broth in each tube 1:10 1 ml 174,000 1:100 1 ml 1 ml 1 ml 1:1000 1 ml 1:10,000 1 ml None of the other four answers (Correct answer not given) 1,000 230,000 174 1 ml 1:100,000 1 mlarrow_forwardDescribe three problems associated with using the standard plate count method for determining the number of bacteria in a sample.arrow_forwardA microbiologist has isolated a new strain of bacteria, and is trying to characterize aspects of its growth. Three flasks of the same media have different amounts of NaCl added. Other growth conditions are identical, and the flasks inoculated at the same time. At regular intervals over a 3 hour period, culture samples are taken and turbidity is measured. Here are the absorbance values over time in a table and graphed. Absorbance (OD600) Time (min) 20% NaCl 3% NaCl 0% NaCl 0 0.05 0.05 0.05 30 0.06 0.05 0.05 60 0.075 0.06 0.05 90 0.11 0.07 0.049 120 0.25 0.1 0.048 150 0.5 0.105 0.055 180 0.7 0.11 0.055 210 0.99 0.15 0.06 240 1.22 0.18 0.055 270 1.35 0.19 0.06 300 1.5 0.2 0.06 330 1.6 0.25 0.062 360 1.7 0.3 0.06 Based on these data, which of the following can be determined about this bacterium? It is psychrophilic. It…arrow_forward
- Which is more Suitable for long-term storage: agar slant or plate? Explain why?arrow_forwardYou spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8) dilution from this same stock of bacteria. Show your work for both.arrow_forwardAnother method of microbial control is filtration. List down at least 5 kinds of filters which could be used for this method?arrow_forward
- Given the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________arrow_forwardThe number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate 1 10 Plate 2 10 Plate 3 10 dilution dilution dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 10 dilution -6 *Too many to count Number of colony forming units (CFU) TMTC* TMTC* 840 28 19 1arrow_forwardThe number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution Plate 3 107 dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* 382 83 10 2 0arrow_forward
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