Brock Biology of Microorganisms (15th Edition)
Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 6, Problem 1AQ

What would happen to regulation from a promoter under negative control if the region where the regulatory protein binds was deleted? What If the promoter was under positive control? Promoters from Escherichia coli under positive control are not close matches to the promoter consensus sequence for E., coll. Why?

Expert Solution
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Summary Introduction

To explain:

What would happen to regulation process from a promoter under negative control if the region where the regulatory protein binds was deleted. What if the promoter was under positive control.

Concept introduction:

The regulation of transcription is effective in negative control, because the cell needs the signal for the presence of enough biosynthetic products for their growth. The transcription is also controlled by both negatively or positively because of the presence of lactose and absence of glucose in the culture medium.

Explanation of Solution

The operonal gene products are produced in high amount when the regulatory protein-binding region of a promoter are deleted under negative control. Because of the repressor protein cannot bind to the promoter region. The operonal gene products are not produced when the regulatory protein-binding region of a promoter are deleted. This is because of the activator which cannot attach itself to the promoter region.

Expert Solution
Check Mark
Summary Introduction

To explain:

Promoters from Escherichia coli under positive control are not close matches to the promoter consensus sequence for E. coli. Why.

Concept introduction:

The regulation of transcription is effective in negative control, because the cell needs the signal for the presence of enough biosynthetic products for their growth. The transcription is also controlled by both negatively or positively because of the presence of lactose and absence of glucose in the culture medium.

Explanation of Solution

The DNA consequences matches closely with the promoter region that leads to the binding of the RNA polymerase to the promoter region and stimulates the transcription without the presence of other factors. Thus, a quite different transcription control is seen in the promoters under positive control.

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Chapter 6 Solutions

Brock Biology of Microorganisms (15th Edition)

Ch. 6.4 - Prob. 2MQCh. 6.4 - Explain how the lac operon is both positively and...Ch. 6.4 - Prob. 1CRCh. 6.5 - What is the major difference between...Ch. 6.5 - How do transcriptional activators in Archaea often...Ch. 6.5 - Explain how the Pyrococcus furiosus TrmBL1...Ch. 6.5 - Prob. 1CRCh. 6.6 - What are kinases and what is their role in...Ch. 6.6 - Prob. 2MQCh. 6.6 - Prob. 1CRCh. 6.7 - What are the primary response regulator and the...Ch. 6.7 - Why is adaptation during chemotaxis important?Ch. 6.7 - How does the response of the chemortaxis system to...Ch. 6.7 - Adaptation allows the mechanism controlling...Ch. 6.8 - What advantage do quorum-sensing systems confer on...Ch. 6.8 - Prob. 2MQCh. 6.8 - Prob. 3MQCh. 6.8 - How can quorum sensing be considered a regulatory...Ch. 6.9 - Which Escherichia coli genes are activated and...Ch. 6.9 - Prob. 2MQCh. 6.9 - What are some other conditions that trigger the...Ch. 6.9 - Explain the sequence of molecular events that...Ch. 6.10 - Prob. 1MQCh. 6.10 - Prob. 2MQCh. 6.10 - Prob. 3MQCh. 6.10 - Prob. 1CRCh. 6.11 - Prob. 1MQCh. 6.11 - Prob. 2MQCh. 6.11 - Prob. 3MQCh. 6.11 - What are the mechanisms by which regulation by...Ch. 6.12 - What happens when a riboswitch binds the small...Ch. 6.12 - What are the major differences between a repressor...Ch. 6.12 - What is the mechanism by which a riboswitch...Ch. 6.13 - Why does attenuation control not occur in...Ch. 6.13 - Prob. 2MQCh. 6.13 - Prob. 1CRCh. 6.14 - What is feedback inhibition?Ch. 6.14 - Prob. 2MQCh. 6.14 - Prob. 3MQCh. 6.14 - Describe how feedback inhibition is reversible.Ch. 6.15 - What types of covalent modifications commonly...Ch. 6.15 - Prob. 2MQCh. 6.15 - Explain the role of an anti-sigma factor.Ch. 6.15 - Which nucleotides are commonly used to covalently...Ch. 6 - What would happen to regulation from a promoter...Ch. 6 - Most of the regulatory systems described in this...Ch. 6 - Many amino acid biosynthetic operons under...Ch. 6 - How would you design a regulatory system to make...
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