Microbiology: Principles and Explorations
9th Edition
ISBN: 9781118743164
Author: Jacquelyn G. Black, Laura J. Black
Publisher: WILEY
expand_more
expand_more
format_list_bulleted
Concept explainers
Textbook Question
Chapter 6, Problem 14SQ
Blood agar is often used to observe changes in the appearance of the agar around the colonies growing on this medium. This medium could then be called:
- (a) Selective
- (b) Designated
- (c) Differential
- (d) Defined
- (e) Exact
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
In this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture?
A) 2.0 X 10^-3 cells/mL
B) 2.0 X 10^5 cells/mL
C) 4.0 X 10^5 cells/mL
D) 5.0 X 10^4 cells/mL
In preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?
A culture of Staphylococcus is diluted as follows:(1) 20mL are added to 80mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-2 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated.
There were 34 colonies counted on one quarter of the plate following incubation.
a) What was the overall dilution?b) How many cfu/mL were present in the original culture?
Chapter 6 Solutions
Microbiology: Principles and Explorations
Ch. 6 - What are the differences between the lag phase and...Ch. 6 - How does logarithmic rate of increase differ from...Ch. 6 - Prob. 1.3SCCh. 6 - Why does a direct microscopic count of bacteria...Ch. 6 - What does the ending -phile mean? Distinguish...Ch. 6 - What enzymes do most obligate anaerobes lack? How...Ch. 6 - Prob. 2.3SCCh. 6 - Prob. 3.1SCCh. 6 - Distinguish between the various kinds of media:...Ch. 6 - What is the purpose of a stock culture? Why is it...
Ch. 6 - Prob. 1CCSCh. 6 - Exactly 100 bacteria with a generation time of 30...Ch. 6 - In the above example, do you think that the number...Ch. 6 - Prob. 3CTQCh. 6 - Prob. 1SQCh. 6 - Match the following growth phase terms to their...Ch. 6 - Which of the following is the best definition of...Ch. 6 - Prob. 4SQCh. 6 - The most probable number (MPN) technique is a...Ch. 6 - Match the terms with their definitions:Ch. 6 - Prob. 7SQCh. 6 - Why do foods containing a high concentration of...Ch. 6 - Some bacteria have complex nutritional...Ch. 6 - Prob. 10SQCh. 6 - Which type of cell will shift to aerobic...Ch. 6 - Which of the following statements about endospores...Ch. 6 - Prob. 13SQCh. 6 - Blood agar is often used to observe changes in the...Ch. 6 - A bacterial medium that contains 20 grams of beef...Ch. 6 - MacConkey agar contains the dye, crystal violet,...Ch. 6 - Prob. 17SQCh. 6 - What are the purposes of carrying out the streak...Ch. 6 - Prob. 19SQCh. 6 - During quorum sensing, bacteria sense their...Ch. 6 - Prob. 21SQCh. 6 - Identify the position of each of the following on...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8) dilution from this same stock of bacteria. Show your work for both.arrow_forwardYou spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8) dilution from this same stock of bacteria.arrow_forwardUsing your fingers, you are asked to aseptically touch the surface of a sterile agar plate. Illustrate the possible result from this step if your fingers are (a) unwashed – touched various things prior to placing on agar surface, and (b) washed with soap or disinfected with 70% alcohol. Describe the relative abundance of microbial growth observed on the plates. List and draw the possible characteristics of an isolated bacterial colony that can be observed based on type of (a) margin, (b) elevation, (c) texture, and (d) optical property.arrow_forward
- How many different types of colonies can you find on the blood agar plate? describe each. (As shown in pictures below) What possible types of bacteria ? (As shown in pictures below)arrow_forwardYou have found that the D-value (decimal reduction value) of an antimicrobial agent to be 4 minutes when the agent was exposed to a bacterial culture having an initial total viable cel1 count of 10° CFU/mL. After 4 minutes upon addition of the antimicrobial agent, what would have been the total viable count of the culture in this experiment? A) O 10° CFU/mL B) O 10$ CFU/mL C) O 10° CFU/mL D) O 10' CFU/mLarrow_forwardIf an OD=1.0 culture yielded a plate count of 2.2 x 107 cfu/mL. What is the cell count of an OD=0.5 culture in cfu/mL? A) 5.5 x 107 B) 2.2 x 106 C) 1.1 x 107 D) 4.4 x 107arrow_forward
- Pick either of these media: (EMB or HE) and describe (again, in your own words) how the chemical ingredients in that medium work to make it both "selective" as well as "differential"arrow_forwardIs the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition. Is the test tube a A) broth, B) slant, or C) deep agar medium? Why is a “needle” used to inoculate?arrow_forwardEthanol has been carried over into your DNA sample (two answers possible here, select both of them) a) The column tip touched the column flow through b) The cells stayed in the lysis buffer for too long c) You didn't add enough wash buffer d) You did not centrifuge a final time after the final washarrow_forward
- There are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one has been incubated for 24 hours. After a 10x dilution by taking 100µl of each culture and adding it to 900 µl water in a microcentrifuge tube and 100µl sample from the tube was taken to view in the counting chamber. a) Count the total number of yeast cells for each culture respectively b) Calculate the concentration and density of yeast cells for each culture respectivelyarrow_forwardduring inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.arrow_forwardAssume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If we can order 5 milligrams of fibronectin for $871.00, how much would it cost to have enough to use every well of the 24-well culture plate?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Molecular Techniques: Basic Concepts; Author: Dr. A's Clinical Lab Videos;https://www.youtube.com/watch?v=7HFHZy8h6z0;License: Standard Youtube License