Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
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Chapter 5.5, Problem 3CC

MAKE CONNECTIONS In Concept 4.7, you learned that animalAnimal cells make an extracellular matrix (ECM). Describe the cellular pathway of synthesis and deposition of an ECM glycoprotein.

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a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?   b) Predict the growth you would expect to see on each of the following plates:         – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?  2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following:  Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng  Final recovery volume: 0.50 mL  Volume plated: 0.25 mL  Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?   2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?   3) Why are the cells incubated at 42°C?

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Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)

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