
Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Textbook Question
Chapter 5.17, Problem 1MQ
Distinguish between the antimicrobial effects of -static, -cidal. and -lytic agents.
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Discussion Questions
Answer these discussion questions and submit them as part of your lab report.
Part A: The Effect of Temperature on Enzyme Activity
Graph the volume of oxygen produced against the temperature of the solution.
How is the oxygen production in 30 seconds related to the rate of the reaction?
At what temperature is the rate of reaction the highest? Lowest? Explain.
Why might the enzyme activity decrease at very high temperatures?
Why might a high fever be dangerous to humans?
What is the optimal temperature for enzymes in the human body?
Part B: The Effect of pH on Enzyme Activity
Graph the volume of oxygen produced against the pH of the solution.
At what pH is the rate of reaction the highest? Lowest? Explain.
Why does changing the pH affect the enzyme activity?
Research the enzyme catalase. What is its function in the human body?
What is the optimal pH for the following enzymes found in the human body? Explain. (catalase, lipase (in your stomach),…
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Discussion Questions:
Part One
Why were the plants kept in the dark prior to the experiment? Why is this important?
Why is it important to boil the leaf?
Explain why it was necessary to use boiling alcohol?
What is the purpose of the iodine?
Part Two
What was the purpose of keeping the leaf in the dark and then covering it with a cardboard cut-out?
What conclusions can you draw from this part of the lab?
Part Three
7. In this experiment what was the purpose of adding the soda lime?
8. Why was a sealed bag placed around each plant?
9. What happened in the control plants?
10. What was the result on photosynthesis?
Part Four
11. Why was a variegated leaf used in this experiment?
!2. What conclusions can you draw about starch production in a variegated leaf?
How did the color differences between the two bacterial species you used in this experiment help you determine if the streak plate method you performed was successful?
Chapter 5 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 5.1 - Define the term generation. What is meant by the...Ch. 5.1 - How do binary fission and budding cell division...Ch. 5.1 - How does the biofilm growth mode differ from that...Ch. 5.1 - Prob. 1CRCh. 5.2 - What is a semilogarithmic plot and what...Ch. 5.2 - For an exponentially growing culture that...Ch. 5.2 - For testing a bacteriums response to a toxic...Ch. 5.2 - How is the generation time (g) of an exponentially...Ch. 5.3 - In which phase of the growth curve do cells divide...Ch. 5.3 - Prob. 2MQ
Ch. 5.3 - Prob. 3MQCh. 5.3 - Describe the growth cycle of a population of...Ch. 5.4 - How do microorganisms in a chemostat differ from...Ch. 5.4 - What happens in a chemostat if the dilution rate...Ch. 5.4 - Do pure cultures have to be used in a chemostat?Ch. 5.4 - How does a chemostat regulate growth rate and cell...Ch. 5.5 - Why would a complex culture medium for Leuconostoc...Ch. 5.5 - In which medium shown in Table 5.1, defined or...Ch. 5.5 - What is meant by the word sterile? Why is aseptic...Ch. 5.5 - How many cells could be present in a single...Ch. 5.5 - Prob. 1CRCh. 5.6 - What are some of the problems that can arise when...Ch. 5.6 - Using microscopic techniques, how could you tell...Ch. 5.6 - Are total cell counts useful if one does not know...Ch. 5.7 - Why is a viable count more sensitive than a...Ch. 5.7 - Describe how you would dilute a bacterial culture...Ch. 5.7 - Prob. 3MQCh. 5.7 - How does a viable count differ from a total count?Ch. 5.8 - List two advantages of using turbidity as a...Ch. 5.8 - Describe how you could use a turbidity measurement...Ch. 5.8 - How can turbidity be used as a measure of cell...Ch. 5.9 - How does a hyperthermophile differ from a...Ch. 5.9 - Prob. 2MQCh. 5.9 - E. coli can grow at a higher temperature in a...Ch. 5.9 - Examine the graph in Figure 5.17. Why is the...Ch. 5.10 - Prob. 1MQCh. 5.10 - What molecular adaptations to cold temperatures...Ch. 5.10 - Prob. 1CRCh. 5.11 - Which phylogenetic domain includes species with...Ch. 5.11 - How does the membrane structure of...Ch. 5.11 - What is Taq polymerase and why is it important?Ch. 5.11 - How do cells of hyperthermophiles prevent heat...Ch. 5.12 - How does the concentration of H+ change when a...Ch. 5.12 - What terms are used to describe organisms whose...Ch. 5.12 - Prob. 3MQCh. 5.12 - Concerning the pH of the environment and of the...Ch. 5.13 - What is the aw of pure water? What is the lower...Ch. 5.13 - What are compatible solutes, and when and why are...Ch. 5.13 - How does a halophile maintain positive water...Ch. 5.14 - How does an obligate aerobe differ from a...Ch. 5.14 - How does a reducing agent work? Give an example of...Ch. 5.14 - How does Superoxide dismutase or superoxide...Ch. 5.14 - Contrast an aerotolerant and an obligate anaerobe...Ch. 5.15 - Why is heat an effective sterilizing agent?Ch. 5.15 - What steps are necessary to ensure the sterility...Ch. 5.15 - Distinguish between the sterilization of...Ch. 5.15 - Contrast the terms thermal death time and decimal...Ch. 5.16 - Define D10 and explain why the killing dose for...Ch. 5.16 - Prob. 2MQCh. 5.16 - Prob. 3MQCh. 5.16 - Prob. 1CRCh. 5.17 - Distinguish between the antimicrobial effects of...Ch. 5.17 - Describe how the minimum inhibitory concentration...Ch. 5.17 - Distinguish between a sterilant, a disinfectant,...Ch. 5.17 - Describe the procedure for obtaining the minimum...Ch. 5 - A medium was inoculated with 5 106 cells/ml of...Ch. 5 - Escherichia coli but not Pyrolobus fumarii will...Ch. 5 - In which direction (into or out of the cell) will...
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