Campbell Biology Plus Mastering Biology with Pearson eText - Access Card Package (11th Edition)
11th Edition
ISBN: 9780134082318
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece
Publisher: PEARSON
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Chapter 50.4, Problem 3CC
Summary Introduction
To predict: Whether a mutation in the mice have acted in the signaling pathway that had disrupted its ability to taste bitter, umami and sweet, but not salty and sour.
Concept introduction: Animals utilize their senses (chemical) for various purpose like recognizing the territory (marked), finding mates and navigating at the time of migration and in many other things. In all the animals, for the purpose of feeding behavior, chemical senses are essential. Perceptions of taste (gustation) depends upon the chemoreceptors.
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Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
Chapter 50 Solutions
Campbell Biology Plus Mastering Biology with Pearson eText - Access Card Package (11th Edition)
Ch. 50.1 - Which one of the five categories of sensory...Ch. 50.1 - Prob. 2CCCh. 50.1 - Prob. 3CCCh. 50.2 - How are otoliths adaptive for burrowing mammals,...Ch. 50.2 - Prob. 2CCCh. 50.2 - Prob. 3CCCh. 50.2 - Prob. 4CCCh. 50.3 - Contrast the light-detecting organs of planarians...Ch. 50.3 - Prob. 2CCCh. 50.3 - Prob. 3CC
Ch. 50.3 - Prob. 4CCCh. 50.4 - Explain why some taste receptor cells arid all...Ch. 50.4 - Prob. 2CCCh. 50.4 - Prob. 3CCCh. 50.5 - Contrast the role of Ca2+ in the contraction of a...Ch. 50.5 - Prob. 2CCCh. 50.5 - Prob. 3CCCh. 50.6 - Contrast swimming and flying in terms of the main...Ch. 50.6 - MAKE CONNECTIONS. Peristalsis contributes to the...Ch. 50.6 - WHAT IF? When using your arms to lower yourself...Ch. 50 - Sensory receptors transduce stimulus energy and...Ch. 50 - How are music volume and pitch encoded in signals...Ch. 50 - Prob. 50.3CRCh. 50 - Prob. 50.4CRCh. 50 - What are two major functions of ATP hydrolysis in...Ch. 50 - Which of the following sensory receptors is...Ch. 50 - The middle ear converts (A) air pressure waves to...Ch. 50 - Prob. 3TYUCh. 50 - Which sensory distinction is not encoded by a...Ch. 50 - The transduction of sound waves into action...Ch. 50 - Although some sharks close their eyes just before...Ch. 50 - Prob. 7TYUCh. 50 - EVOLUTION CONNECTION In general, locomotion on...Ch. 50 - Prob. 9TYUCh. 50 - WRITE ABOUT A THEME: ORGANIZATION In a short essay...Ch. 50 - SYNTHESIZE YOUR KNOWLEDGE Bloodhounds, which are...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forward
- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have shipped your samples off for sequencing and are now waiting for the results. Out of curiosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidase enzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you do see a color change to purple during the Oxidase test. What results can you conclude from this? O Catalase-/ Oxidase + O Catalase +/ Oxidase + Catalase + / Oxidase- O Catalase / Oxidase - O None of the abovearrow_forwardWhich of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forwardWhy are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forward
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