Campbell Biology Plus Mastering Biology with Pearson eText - Access Card Package (11th Edition)
11th Edition
ISBN: 9780134082318
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece
Publisher: PEARSON
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Chapter 50, Problem 8TYU
EVOLUTION CONNECTION In general, locomotion on land will require more energy than locomotion in water. By integrating what you learned about animal form and function in Unit 7, discuss some of the evolutionary adaptations of mammals that support the high energy requirements for moving on land.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 50 Solutions
Campbell Biology Plus Mastering Biology with Pearson eText - Access Card Package (11th Edition)
Ch. 50.1 - Which one of the five categories of sensory...Ch. 50.1 - Prob. 2CCCh. 50.1 - Prob. 3CCCh. 50.2 - How are otoliths adaptive for burrowing mammals,...Ch. 50.2 - Prob. 2CCCh. 50.2 - Prob. 3CCCh. 50.2 - Prob. 4CCCh. 50.3 - Contrast the light-detecting organs of planarians...Ch. 50.3 - Prob. 2CCCh. 50.3 - Prob. 3CC
Ch. 50.3 - Prob. 4CCCh. 50.4 - Explain why some taste receptor cells arid all...Ch. 50.4 - Prob. 2CCCh. 50.4 - Prob. 3CCCh. 50.5 - Contrast the role of Ca2+ in the contraction of a...Ch. 50.5 - Prob. 2CCCh. 50.5 - Prob. 3CCCh. 50.6 - Contrast swimming and flying in terms of the main...Ch. 50.6 - MAKE CONNECTIONS. Peristalsis contributes to the...Ch. 50.6 - WHAT IF? When using your arms to lower yourself...Ch. 50 - Sensory receptors transduce stimulus energy and...Ch. 50 - How are music volume and pitch encoded in signals...Ch. 50 - Prob. 50.3CRCh. 50 - Prob. 50.4CRCh. 50 - What are two major functions of ATP hydrolysis in...Ch. 50 - Which of the following sensory receptors is...Ch. 50 - The middle ear converts (A) air pressure waves to...Ch. 50 - Prob. 3TYUCh. 50 - Which sensory distinction is not encoded by a...Ch. 50 - The transduction of sound waves into action...Ch. 50 - Although some sharks close their eyes just before...Ch. 50 - Prob. 7TYUCh. 50 - EVOLUTION CONNECTION In general, locomotion on...Ch. 50 - Prob. 9TYUCh. 50 - WRITE ABOUT A THEME: ORGANIZATION In a short essay...Ch. 50 - SYNTHESIZE YOUR KNOWLEDGE Bloodhounds, which are...
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