BIOLOGY 12TH ED (LL) W/ CONNECT
12th Edition
ISBN: 9781264898091
Author: Raven
Publisher: MCG
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Chapter 50.1, Problem 2LO
Summary Introduction
To explain: How pathogens are recognized by the innate immune system.
Introduction: For a long time, the immune response of vertebrates to the pathogenic invasion was categorized into the specific and non-specific mode of defense. Now, it is clear that it is not an exact view of the immune system. Thus, we now classify the immune system into two portions: innate and adaptive immunity.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 50 Solutions
BIOLOGY 12TH ED (LL) W/ CONNECT
Ch. 50.1 - Prob. 1LOCh. 50.1 - Prob. 2LOCh. 50.1 - Prob. 3LOCh. 50.2 - Prob. 1LOCh. 50.2 - Prob. 2LOCh. 50.2 - Prob. 3LOCh. 50.3 - Prob. 1LOCh. 50.3 - Prob. 2LOCh. 50.4 - Explain how antibody diversity is generated.Ch. 50.4 - Prob. 2LO
Ch. 50.4 - Prob. 3LOCh. 50.5 - Define autoimmune diseases.Ch. 50.5 - Prob. 2LOCh. 50.6 - Explain antigenantibody reactions in the ABO blood...Ch. 50.6 - Prob. 2LOCh. 50.6 - Prob. 3LOCh. 50.7 - Prob. 1LOCh. 50.7 - Prob. 2LOCh. 50 - How would a high level of HCG present in a urine...Ch. 50 - What would happen if an NK cell killed a virally...Ch. 50 - Prob. 2IQCh. 50 - Prob. 3IQCh. 50 - Prob. 4IQCh. 50 - Cells that target and kill body cells infected by...Ch. 50 - Structures on invading cells recognized by the...Ch. 50 - Which one of the following acts as the alarm...Ch. 50 - Prob. 4UCh. 50 - Receptors that trigger innate immune responses a....Ch. 50 - Diseases in which the persons immune system no...Ch. 50 - Suppose that a new disease is discovered that...Ch. 50 - You start a new job in a research lab. The lab...Ch. 50 - In comparing T-cell receptors and immunoglobulins...Ch. 50 - If you have type AB blood, which of the following...Ch. 50 - Suppose that you get a paper cut while studying....Ch. 50 - If you wanted to cure allergies by bioengineering...Ch. 50 - Why do we need to be repeatedly vaccinated for...Ch. 50 - Prob. 7ACh. 50 - Suppose you take a job in the marketing department...Ch. 50 - Prob. 2SCh. 50 - Prob. 3SCh. 50 - Toll-like receptors have been found in a wide...
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