HUMAN ANATOMY
6th Edition
ISBN: 9781260986037
Author: SALADIN
Publisher: MCG
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Chapter 5, Problem 3TYC
Summary Introduction
To analyze:
The unity and functions of the two epidermal layers.
Introduction : Collagen makes up the majority of the dermis, which is a type of connective tissue. Dermis plays a vital role in body temperature regulation.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 5 Solutions
HUMAN ANATOMY
Ch. 5.1 - Dermal papillae are relatively high and numerous...Ch. 5.1 - An infant brought to a clinic shows abnormally...Ch. 5.1 - Prob. 1BYGOCh. 5.1 - Prob. 2BYGOCh. 5.1 - List the five cell types of the epidermis....Ch. 5.1 - Prob. 4BYGOCh. 5.1 - What are the two layers of the dermis? What type...Ch. 5.1 - Name the pigments responsible for normal skin...Ch. 5.2 - Prob. 7BYGOCh. 5.2 - Prob. 8BYGO
Ch. 5.2 - Prob. 9BYGOCh. 5.2 - Prob. 10BYGOCh. 5.2 - Describe some similarities between a nail and a...Ch. 5.3 - Prob. 12BYGOCh. 5.3 - What types of hair are associated with apocrine...Ch. 5.3 - Prob. 14BYGOCh. 5.3 - What is the difference between a breast and...Ch. 5.4 - Prob. 1AWYKCh. 5.4 - What adult skin laver arises from the germinative...Ch. 5.4 - Prob. 17BYGOCh. 5.4 - What types of cells are involved in each type of...Ch. 5.4 - Which type of skin cancer is most dangerous? What...Ch. 5.4 - What is the difference between a first-, second-,...Ch. 5 - The difference between the integumentary system...Ch. 5 - Prob. 5.1.2AYLOCh. 5 - The range of thicknesses of the skin, the basis...Ch. 5 - Prob. 5.1.4AYLOCh. 5 - The five epidermal cell types and their respective...Ch. 5 - The four to five strata seen in thin and thick...Ch. 5 - Prob. 5.1.7AYLOCh. 5 - Prob. 5.1.8AYLOCh. 5 - Prob. 5.1.9AYLOCh. 5 - Prob. 5.1.10AYLOCh. 5 - Prob. 5.1.11AYLOCh. 5 - Prob. 5.1.12AYLOCh. 5 - The histological composition of the hypodermis and...Ch. 5 - Prob. 5.1.14AYLOCh. 5 - Prob. 5.1.15AYLOCh. 5 - The various kinds of lines, creases, and other...Ch. 5 - Prob. 5.2.1AYLOCh. 5 - Prob. 5.2.2AYLOCh. 5 - Prob. 5.2.3AYLOCh. 5 - Prob. 5.2.4AYLOCh. 5 - Prob. 5.2.5AYLOCh. 5 - Prob. 5.2.6AYLOCh. 5 - Prob. 5.2.7AYLOCh. 5 - Prob. 5.2.8AYLOCh. 5 - Prob. 5.2.9AYLOCh. 5 - Prob. 5.2.10AYLOCh. 5 - Types of hair thinning and factors that contribute...Ch. 5 - Prob. 5.2.12AYLOCh. 5 - The two types of sweat glands and how they differ...Ch. 5 - Prob. 5.3.2AYLOCh. 5 - Prob. 5.3.3AYLOCh. 5 - Prob. 5.3.4AYLOCh. 5 - Prob. 5.4.1AYLOCh. 5 - Prob. 5.4.2AYLOCh. 5 - Prob. 5.4.3AYLOCh. 5 - How the two types of sweat glands differ in their...Ch. 5 - Prob. 5.4.5AYLOCh. 5 - Prob. 5.4.6AYLOCh. 5 - Prob. 5.4.7AYLOCh. 5 - Prob. 1TYRCh. 5 - Prob. 2TYRCh. 5 - Prob. 3TYRCh. 5 - All of the following interfere with microbial...Ch. 5 - Prob. 5TYRCh. 5 - Prob. 6TYRCh. 5 - Prob. 7TYRCh. 5 - Prob. 8TYRCh. 5 - Prob. 9TYRCh. 5 - Prob. 10TYRCh. 5 - Prob. 11TYRCh. 5 - Prob. 12TYRCh. 5 - The most abundant protein of the epidermis is...Ch. 5 - Blueness of the skin due to low oxygen...Ch. 5 - Projections of the dermis toward the epidermis are...Ch. 5 - Cerumen is more commonly known as _____________.Ch. 5 - The holocrine glands that secret into a hair...Ch. 5 - The scaly outermost layer of a hair is called the...Ch. 5 - Prob. 19TYRCh. 5 - Prob. 20TYRCh. 5 - Prob. 1BYMVCh. 5 - Prob. 2BYMVCh. 5 - Prob. 3BYMVCh. 5 - Prob. 4BYMVCh. 5 - Prob. 5BYMVCh. 5 - Prob. 6BYMVCh. 5 - Prob. 7BYMVCh. 5 - Prob. 8BYMVCh. 5 - Prob. 9BYMVCh. 5 - State a meaning of each word element and give a...Ch. 5 - Prob. 1WWWTSCh. 5 - Prob. 2WWWTSCh. 5 - Prob. 3WWWTSCh. 5 - Prob. 4WWWTSCh. 5 - Briefly explain why each of the following...Ch. 5 - Prob. 6WWWTSCh. 5 - Prob. 7WWWTSCh. 5 - Prob. 8WWWTSCh. 5 - Prob. 9WWWTSCh. 5 - Prob. 10WWWTSCh. 5 - Many organs of the body contain numerous smaller...Ch. 5 - Prob. 2TYCCh. 5 - Prob. 3TYCCh. 5 - Prob. 4TYCCh. 5 - Prob. 5TYC
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