Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 5, Problem 2TQ
Summary Introduction
To review:
The process for the conversion of a facultative anaerobe into an obligate anaerobe.
Introduction:
Certain microbes can grow in the presence of oxygen while others require an environment which is totally devoid of oxygen. The microbes that grow in the presence of oxygen are termed as aerobes while the microbes that survive in the absence of oxygen are known as anaerobes. Anaerobes generate energy through the fermentation process while aerobes produce energy through
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 5 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 5.2 - Prob. 1TQCh. 5.2 - Prob. 2TQCh. 5.2 - Prob. 3TQCh. 5.3 - Prob. 1TQCh. 5.4 - Prob. 1TQCh. 5.5 - Prob. 1TQCh. 5.5 - Prob. 2TQCh. 5.5 - Prob. 3TQCh. 5.6 - Prob. 1TQCh. 5.7 - Prob. 1TQ
Ch. 5.7 - Prob. 2TQCh. 5.7 - Prob. 3TQCh. 5 - Prob. 1RQCh. 5 - Prob. 2RQCh. 5 - Prob. 3RQCh. 5 - Prob. 4RQCh. 5 - Prob. 5RQCh. 5 - Prob. 6RQCh. 5 - Prob. 7RQCh. 5 - Prob. 8RQCh. 5 - Prob. 9RQCh. 5 - Prob. 10RQCh. 5 - Prob. 11RQCh. 5 - Prob. 12RQCh. 5 - Prob. 1TQCh. 5 - Prob. 2TQCh. 5 - Prob. 3TQCh. 5 - Prob. 4TQCh. 5 - Prob. 5TQ
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