Whether the lack of a strong reaction on the EMB plate refutes the determination that the isolated bacteria are an E. coli culture. Introduction: Bacteria are the unicellular prokaryotic organisms that maintain a definite shape since they contain a rigid cell wall. Based on gram staining , they are divided into two types namely gram-positive bacteria and gram-negative bacteria. Escherichia coli is the gram negative, motile, rod-shaped, enteric bacterium that is usually found in the human intestine.

Question

Want to see more full solutions like this?

Answer 1

Question

Chapter 5, Problem 1QC

Summary Introduction

To explain: Whether the lack of a strong reaction on the EMB plate refutes the determination that the isolated bacteria are an E. coli culture.

Introduction: Bacteria are the unicellular prokaryotic organisms that maintain a definite shape since they contain a rigid cell wall. Based on gram staining, they are divided into two types namely gram-positive bacteria and gram-negative bacteria. Escherichia coli is the gram negative, motile, rod-shaped, enteric bacterium that is usually found in the human intestine.

Expert Solution & Answer

Explanation of Solution

Eosin Methylene Blue (EMB) agar medium is generally used for the examination of E. coli. This medium acts as both selective and differential medium that is used to differentiate between coliforms and fecal coliforms. Since E. coli is a lactose fermenter, it ferments the lactose present in the EMB agar medium and produces metallic green color in the medium, and other non-lactose fermenters appear as colorless. Thus, the lack of strong reaction on the EMB medium plate refutes the determination that the isolated bacteria are an E. coli culture. However, other biochemical tests can be used to confirm the presence or absence of E.coli in the medium.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

I have a question. I need to make 25 mL of this solution . How would I calculate the math? Please help

Introduction to blood lab report

Which of the structural components listed in the Essential terms of section 1.3 (Cell components) could occur in a plant cell? Paragraph く BIUA 川くく 80 + кл K

Answer 2

Question

Chapter 5, Problem 1QC

Summary Introduction

To explain: Whether the lack of a strong reaction on the EMB plate refutes the determination that the isolated bacteria are an E. coli culture.

Introduction: Bacteria are the unicellular prokaryotic organisms that maintain a definite shape since they contain a rigid cell wall. Based on gram staining, they are divided into two types namely gram-positive bacteria and gram-negative bacteria. Escherichia coli is the gram negative, motile, rod-shaped, enteric bacterium that is usually found in the human intestine.

Expert Solution & Answer

Explanation of Solution

Eosin Methylene Blue (EMB) agar medium is generally used for the examination of E. coli. This medium acts as both selective and differential medium that is used to differentiate between coliforms and fecal coliforms. Since E. coli is a lactose fermenter, it ferments the lactose present in the EMB agar medium and produces metallic green color in the medium, and other non-lactose fermenters appear as colorless. Thus, the lack of strong reaction on the EMB medium plate refutes the determination that the isolated bacteria are an E. coli culture. However, other biochemical tests can be used to confirm the presence or absence of E.coli in the medium.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Question

Chapter 5, Problem 1QC

Summary Introduction

To explain: Whether the lack of a strong reaction on the EMB plate refutes the determination that the isolated bacteria are an E. coli culture.

Introduction: Bacteria are the unicellular prokaryotic organisms that maintain a definite shape since they contain a rigid cell wall. Based on gram staining, they are divided into two types namely gram-positive bacteria and gram-negative bacteria. Escherichia coli is the gram negative, motile, rod-shaped, enteric bacterium that is usually found in the human intestine.

Expert Solution & Answer

Explanation of Solution

Eosin Methylene Blue (EMB) agar medium is generally used for the examination of E. coli. This medium acts as both selective and differential medium that is used to differentiate between coliforms and fecal coliforms. Since E. coli is a lactose fermenter, it ferments the lactose present in the EMB agar medium and produces metallic green color in the medium, and other non-lactose fermenters appear as colorless. Thus, the lack of strong reaction on the EMB medium plate refutes the determination that the isolated bacteria are an E. coli culture. However, other biochemical tests can be used to confirm the presence or absence of E.coli in the medium.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Question

Chapter 5, Problem 1QC

Summary Introduction

To explain: Whether the lack of a strong reaction on the EMB plate refutes the determination that the isolated bacteria are an E. coli culture.

Introduction: Bacteria are the unicellular prokaryotic organisms that maintain a definite shape since they contain a rigid cell wall. Based on gram staining, they are divided into two types namely gram-positive bacteria and gram-negative bacteria. Escherichia coli is the gram negative, motile, rod-shaped, enteric bacterium that is usually found in the human intestine.

Expert Solution & Answer

Explanation of Solution

Eosin Methylene Blue (EMB) agar medium is generally used for the examination of E. coli. This medium acts as both selective and differential medium that is used to differentiate between coliforms and fecal coliforms. Since E. coli is a lactose fermenter, it ferments the lactose present in the EMB agar medium and produces metallic green color in the medium, and other non-lactose fermenters appear as colorless. Thus, the lack of strong reaction on the EMB medium plate refutes the determination that the isolated bacteria are an E. coli culture. However, other biochemical tests can be used to confirm the presence or absence of E.coli in the medium.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Chapter 5, Problem 1QC

Summary Introduction

To explain: Whether the lack of a strong reaction on the EMB plate refutes the determination that the isolated bacteria are an E. coli culture.

Introduction: Bacteria are the unicellular prokaryotic organisms that maintain a definite shape since they contain a rigid cell wall. Based on gram staining, they are divided into two types namely gram-positive bacteria and gram-negative bacteria. Escherichia coli is the gram negative, motile, rod-shaped, enteric bacterium that is usually found in the human intestine.

Expert Solution & Answer

Explanation of Solution

Eosin Methylene Blue (EMB) agar medium is generally used for the examination of E. coli. This medium acts as both selective and differential medium that is used to differentiate between coliforms and fecal coliforms. Since E. coli is a lactose fermenter, it ferments the lactose present in the EMB agar medium and produces metallic green color in the medium, and other non-lactose fermenters appear as colorless. Thus, the lack of strong reaction on the EMB medium plate refutes the determination that the isolated bacteria are an E. coli culture. However, other biochemical tests can be used to confirm the presence or absence of E.coli in the medium.

Answer 6

Chapter 5, Problem 1QC

Summary Introduction

To explain: Whether the lack of a strong reaction on the EMB plate refutes the determination that the isolated bacteria are an E. coli culture.

Introduction: Bacteria are the unicellular prokaryotic organisms that maintain a definite shape since they contain a rigid cell wall. Based on gram staining, they are divided into two types namely gram-positive bacteria and gram-negative bacteria. Escherichia coli is the gram negative, motile, rod-shaped, enteric bacterium that is usually found in the human intestine.

Expert Solution & Answer

Explanation of Solution

Eosin Methylene Blue (EMB) agar medium is generally used for the examination of E. coli. This medium acts as both selective and differential medium that is used to differentiate between coliforms and fecal coliforms. Since E. coli is a lactose fermenter, it ferments the lactose present in the EMB agar medium and produces metallic green color in the medium, and other non-lactose fermenters appear as colorless. Thus, the lack of strong reaction on the EMB medium plate refutes the determination that the isolated bacteria are an E. coli culture. However, other biochemical tests can be used to confirm the presence or absence of E.coli in the medium.

Answer 7

Chapter 5, Problem 1QC

Summary Introduction

To explain: Whether the lack of a strong reaction on the EMB plate refutes the determination that the isolated bacteria are an E. coli culture.

Introduction: Bacteria are the unicellular prokaryotic organisms that maintain a definite shape since they contain a rigid cell wall. Based on gram staining, they are divided into two types namely gram-positive bacteria and gram-negative bacteria. Escherichia coli is the gram negative, motile, rod-shaped, enteric bacterium that is usually found in the human intestine.

Answer 8

Expert Solution & Answer

Explanation of Solution

Eosin Methylene Blue (EMB) agar medium is generally used for the examination of E. coli. This medium acts as both selective and differential medium that is used to differentiate between coliforms and fecal coliforms. Since E. coli is a lactose fermenter, it ferments the lactose present in the EMB agar medium and produces metallic green color in the medium, and other non-lactose fermenters appear as colorless. Thus, the lack of strong reaction on the EMB medium plate refutes the determination that the isolated bacteria are an E. coli culture. However, other biochemical tests can be used to confirm the presence or absence of E.coli in the medium.