Microbiology: A Laboratory Manual, Loose Leaf Edition (12th Edition)
Microbiology: A Laboratory Manual, Loose Leaf Edition (12th Edition)
12th Edition
ISBN: 9780135188996
Author: James G. Cappuccino, Chad T. Welsh
Publisher: PEARSON
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Chapter 5, Problem 31E.1RQ
Summary Introduction

To explain: The reason for the development of yellow color in the medium which is an indication of the negative decarboxylase test.

Introduction: Decarboxylation is a chemical reaction which involves the removal of the carboxyl group and the release of carbon dioxide. This is carried out by the microbes possessing decarboxylase enzymes that are specific to the amino acid substrates on which they act. In this process, the microbes are cultivated in an acid environment along with the presence of a specific substrate. The end product of this results in a shift to an alkaline environment.

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Microbiology: A Laboratory Manual, Loose Leaf Edition (12th Edition)

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