FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
5th Edition
ISBN: 9781119661450
Author: Voet
Publisher: WILEY
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Chapter 5, Problem 17E
Summary Introduction
To explain: The reason why valine is rarely used as a substitute for glycine in site-directed mutagenesis.
Concept introduction: Site-directed mutagenesis is the method, which is used to make internal changes to the DNA sequences of gene and gene products. It is also used to study the protein function, identifying the active sites, and design novel proteins in drug discovery.
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Chapter 5 Solutions
FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
Ch. 5 - Prob. 1ECh. 5 - Prob. 2ECh. 5 - 3. Which peptide has greater absorbance at 280...Ch. 5 - 4. Protein X has an absorptivity of 0.4 mL · mg?1...Ch. 5 - 5. You are using ammonium sulfate to purify...Ch. 5 - Prob. 6ECh. 5 - 7. (a) In what order would the amino acids Arg,...Ch. 5 - Prob. 8ECh. 5 - 9. Explain why a certain protein has an apparent...Ch. 5 - 10. Determine the subunit composition of a protein...
Ch. 5 - 11. Explain why a protein, which has a...Ch. 5 - 12. A protein has an apparent mass of 800 kD by...Ch. 5 - 13. Explain why the dansyl chloride treatment of a...Ch. 5 - 14. Identify the tint residue obtained by Edman...Ch. 5 - 15. A pentapeptide has the sequence NNKNN (using...Ch. 5 - 16. The peptide in Problem 15 was sequenced by...Ch. 5 - 17. In site-directed mutagenesis experiments, Gly...Ch. 5 - 18. Below is a list of the first 10 residues of...Ch. 5 - Prob. 19ECh. 5 - Prob. 20ECh. 5 - Prob. 21CQCh. 5 - Prob. 22CQCh. 5 - 23. What fractionation procedure could be used to...Ch. 5 - Prob. 24CQCh. 5 - 25. Purification tables are often used to keep...Ch. 5 - Prob. 26CQCh. 5 - 27. You must cleave the following peptide into...Ch. 5 - 28. You wish to determine the sequence of a...Ch. 5 - Prob. 29CQCh. 5 - Prob. 30CQCh. 5 - 31. You wish to determine the sequence of a short...Ch. 5 - Prob. 32CQCh. 5 - Prob. 33CQCh. 5 - 34. You wish to sequence the light chain of a...Ch. 5 - Prob. 1MTE
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