FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
5th Edition
ISBN: 9781119661450
Author: Voet
Publisher: WILEY
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Chapter 5, Problem 3E
Summary Introduction
Concept introduction: Absorbance is the measurement of the amount of light that a substance absorbs at a particular wavelength of light. Absorbance is measured using a spectrophotometer. Proteins absorb ultraviolet (UV) light approximately at 280nm. The UV absorption of protein used to identify the spectra of the microscopic samples. The spectra of the protein sample are used to identify the protein concentration and relative amount of protein.
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Chapter 5 Solutions
FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
Ch. 5 - Prob. 1ECh. 5 - Prob. 2ECh. 5 - 3. Which peptide has greater absorbance at 280...Ch. 5 - 4. Protein X has an absorptivity of 0.4 mL · mg?1...Ch. 5 - 5. You are using ammonium sulfate to purify...Ch. 5 - Prob. 6ECh. 5 - 7. (a) In what order would the amino acids Arg,...Ch. 5 - Prob. 8ECh. 5 - 9. Explain why a certain protein has an apparent...Ch. 5 - 10. Determine the subunit composition of a protein...
Ch. 5 - 11. Explain why a protein, which has a...Ch. 5 - 12. A protein has an apparent mass of 800 kD by...Ch. 5 - 13. Explain why the dansyl chloride treatment of a...Ch. 5 - 14. Identify the tint residue obtained by Edman...Ch. 5 - 15. A pentapeptide has the sequence NNKNN (using...Ch. 5 - 16. The peptide in Problem 15 was sequenced by...Ch. 5 - 17. In site-directed mutagenesis experiments, Gly...Ch. 5 - 18. Below is a list of the first 10 residues of...Ch. 5 - Prob. 19ECh. 5 - Prob. 20ECh. 5 - Prob. 21CQCh. 5 - Prob. 22CQCh. 5 - 23. What fractionation procedure could be used to...Ch. 5 - Prob. 24CQCh. 5 - 25. Purification tables are often used to keep...Ch. 5 - Prob. 26CQCh. 5 - 27. You must cleave the following peptide into...Ch. 5 - 28. You wish to determine the sequence of a...Ch. 5 - Prob. 29CQCh. 5 - Prob. 30CQCh. 5 - 31. You wish to determine the sequence of a short...Ch. 5 - Prob. 32CQCh. 5 - Prob. 33CQCh. 5 - 34. You wish to sequence the light chain of a...Ch. 5 - Prob. 1MTE
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