To derive: The degree of purification for a purified fraction. Concept introduction: Sedimentation coefficient of a particle is used to characterize its behavior in the sedimentation processes, usually centrifugation. Ultracentrifugation is a technique that involves the application of centrifugal force to separate molecules from the solution, based on their viscosity of the medium, shape, density, size, and the rotor speed. Ammonium sulfate precipitation is the most common and effective method for fractionating proteins.

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Chapter 5, Problem 26CQ

(a)

Summary Introduction

To derive: The degree of purification for a purified fraction.

Concept introduction: Sedimentation coefficient of a particle is used to characterize its behavior in the sedimentation processes, usually centrifugation. Ultracentrifugation is a technique that involves the application of centrifugal force to separate molecules from the solution, based on their viscosity of the medium, shape, density, size, and the rotor speed. Ammonium sulfate precipitation is the most common and effective method for fractionating proteins.

(b)

Summary Introduction

To derive: The percent of yield of enzyme recovered from the purified fraction.

Concept introduction: Sedimentation coefficient of a particle is used to characterize its behavior in the sedimentation processes, usually centrifugation. Ultracentrifugation is a technique that involves the application of centrifugal force to separate molecules from the solution, based on their viscosity of the medium, shape, density, size, and the rotor speed. Ammonium sulfate precipitation is the most common and effective method for fractionating proteins.

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