Quantitative Chemical Analysis
Quantitative Chemical Analysis
9th Edition
ISBN: 9781464135385
Author: Daniel C. Harris
Publisher: W. H. Freeman
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Chapter 4.8, Problem 9TY
Interpretation Introduction

Interpretation:

The mass of protein that gives a corrected absorbance of 0.250 has to be calculated.

Concept Introduction:

Linear Calibration Curve:

The equation of linear calibration line is:

y(±sy)=[m(±um)]x+[b(±ub)]

Where,

y is the corrected absorbance.

corrected absorbance = observed absorbance - blank absorbance

To Calculate: The mass of protein that gives a corrected absorbance of 0.250

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