Campbell Biology Custom Stony Brook 10 Th Edition
10th Edition
ISBN: 9781269870818
Author: Reece Urry Cain Wasserman Minorsky Jackson
Publisher: PEARSON
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Chapter 47, Problem 47.2CR
Summary Introduction
To explain: The formation of neural tube and neural crest cells.
Concept introduction: Neural tube is an embryonic structure which is found in the vertebrate embryo. These neural tubes can ultimately form the brain and spinal cord. The neural tube is formed by a process called neurulation.
Neural crest cells are a group of cells which are originated at the dorsal most region of the neural tube. The neural crest cells can migrate to give rise to a number of differentiated cell types. These include the neurons, bone, pigment cells, facial skeleton, cartilage, glia, peripheral nervous system and facial skeleton.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
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Add CaCl2 to both tubes.
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Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 47 Solutions
Campbell Biology Custom Stony Brook 10 Th Edition
Ch. 47.1 - How does the fertilization envelope form in sea...Ch. 47.1 - Prob. 2CCCh. 47.1 - MAKE CONNECTIONS Review Figure 12.16 on cell...Ch. 47.2 - In the frog embryo, convergent extension elongates...Ch. 47.2 - WHAT IF? Predict what would happen if Ca2+ was...Ch. 47.2 - MAKE CONNECTIONS Unlike some other types of birth...Ch. 47.3 - Prob. 1CCCh. 47.3 - Prob. 2CCCh. 47.3 - Prob. 3CCCh. 47.3 - Prob. 4CC
Ch. 47 - What cell-surface event would likely fail if a...Ch. 47 - Prob. 47.2CRCh. 47 - Suppose you found two classes of mouse mutations,...Ch. 47 - Prob. 1TYUCh. 47 - Which of the following is common to the...Ch. 47 - The archenteron develops into a. the mesoderm. b....Ch. 47 - What structural adaptation in chickens allows them...Ch. 47 - Prob. 5TYUCh. 47 - In humans, identical twins are possible because a....Ch. 47 - Cells transplanted from the neural tube of a frog...Ch. 47 - DRAW IT Each blue circle in the figure below...Ch. 47 - EVOLUTION CONNECTION Evolution in insects and...Ch. 47 - Prob. 10TYUCh. 47 - Prob. 11TYUCh. 47 - Prob. 12TYUCh. 47 - SYNTHESIZE YOUR KNOWLEDGE Occasionally, two-headed...
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