BIOLOGY(LL)-W/ACCESS CODE >CUSTOM<
12th Edition
ISBN: 9781264058167
Author: Raven
Publisher: MCG CUSTOM
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Chapter 45, Problem 2A
You have identified a calcium storage disease in rats. How would this inability to store Ca2+ affect muscle contraction?
a. Ca2+ would be unable to bind to tropomyosin, which enables troponin to move and reveal binding sites for cross-bridges.
b. Ca2+ would be unable to bind to troponin, which enables tropomyosin to move and reveal binding sites for cross-bridges.
c. Ca2+ would be unable to bind to tropomyosin, which enables troponin to release ATP.
d. Ca2+ would be unable to bind to troponin, which enables tropomyosin to release ATP.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 45 Solutions
BIOLOGY(LL)-W/ACCESS CODE >CUSTOM<
Ch. 45.1 - Compare hydrostatic skeletons, exoskeletons, and...Ch. 45.1 - Explain how animals with hydrostatic skeletons...Ch. 45.2 - Prob. 1LOCh. 45.2 - Prob. 2LOCh. 45.2 - Prob. 3LOCh. 45.3 - Prob. 1LOCh. 45.4 - Prob. 1LOCh. 45.4 - Prob. 2LOCh. 45.4 - Prob. 3LOCh. 45.5 - Prob. 1LO
Ch. 45.5 - Describe how friction and gravity affect...Ch. 45.5 - Prob. 3LOCh. 45 - Prob. 1IQCh. 45 - Exoskeletons and endoskeletons differ in that a....Ch. 45 - Worms use a hydrostatic skeleton to generate...Ch. 45 - Bone develops by one of two mechanisms depending...Ch. 45 - Which of the following statements best describes...Ch. 45 - Motor neurons stimulate muscle contraction via the...Ch. 45 - Prob. 6UCh. 45 - If you wanted to study the use of ATP during a...Ch. 45 - Place the following events in the correct order....Ch. 45 - You take X-rays of two individuals. Ray has been a...Ch. 45 - You have identified a calcium storage disease in...Ch. 45 - How do the muscles move your hand through space?...Ch. 45 - How can osteocytes remain alive within bone? a....Ch. 45 - Swimming underwater using forelimbs for propulsion...Ch. 45 - If a drug inhibits the release of ACh, what will...Ch. 45 - Prob. 1SCh. 45 - Prob. 2SCh. 45 - The nerve gas methylphosphonofluoridic acid...Ch. 45 - If natural selection favors the evolution of wings...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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