Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 43, Problem 1Q
What was the antiserum used in this experiment?
Expert Solution & Answer
Summary Introduction
To fill:
Patient's serum
Introduction:
Agglutination is the phenomenon by which the antibody gets clumped to the antigenic components like flagellum, cell walls or capsules that are found on the cells of the bacterium. An unknown bacterium is usually mixed in saline and taken in a slide and antiserum containing known antibodies is added. Agglutination happens if the antibodies encounter antigens of the same species or strains as that of the antibody.
Answer to Problem 1Q
The patients serum is the antiserum used in this experiment.
Explanation of Solution
In the experiment, initially patient's serum is mixed with saline and wells are labeled and then dilutions of the antiserum( patient's serum) is made .Then to it antigen was added .The endpoint or the titer is the highest dilution in which clumping has taken place clearly. The patient's serum contains antibodies to the Salmonella entericaTyphimurium if clumping is detected. If there is no clumping or agglutination ,then there are no antibodies to the above mentioned bacterium. This forms the basis for Widal test or test for diagnosis of Typhoid fever.
Want to see more full solutions like this?
Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!
Students have asked these similar questions
what measures can a mother to take to improve the produce of her to milk to her newborn baby ?
1. Color the line that represents all ancestors of the Eastern white pine tree green (but only the ancestral line NOT shared with other organisms)
2. Oncle the last common ancestor of the Colorado blue spruce tree and Eastern white pine tree.
3. Put a box around the last common ancestor of the sugar maple tree and the dogwood tree.
4. Put a triangle around the last common ancestor of the red pine tree and the american holly bush.
5. Color the line that represents all ancestors of the Ponderosa pine tree red (including all shared ancestors).
6. Color the line that represents all ancestors of the American elm tree blue (including all shared ancestors).
7 Color the line that represents all ancestors of the Sabal palm tree purple (including all shared ancestors)
8. Using a yellow highlighter or colored pencil, circle the clade that includes all pine trees.
9. Using a orange highlighter or colored pencil, circle the clade that includes all gymnosperms
10. Can you tell…
You have been hired as a public relations specialist to give invertebrates a good name. After all, they are much more than just creepy crawly bugs!
Your first task though is to convince yourself that is true. The best way to do that is to start close to home. Find something in your house that is a product obtained directly from an invertebrate or only due to an invertebrate’s actions. Describe the product, its function and utility, as well as any human manufactured alternatives. Be sure to highlight the advantages of obtaining this directly from nature.
Keep in mind, a product can be something you use, wear, eat, or enjoy for its visual appeal.
Chapter 43 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Use the following tree diagram to answer Questions #8-10. 8) Which of the following two animals are the most closely related based on the tree to the left? a) Pig and camel b) Hippo and pig c) Deer and cow 9) CIRCLE on the tree diagram where the common ancestor between a hippo and a cow is. 10) Put a SQUARE on the tree diagram where the common ancestor between a pig and a peccary is.arrow_forwardExplain: Healthy Cell Function Overview→ Briefly describe how a healthy cell usually works: metabolism (ATP production), pH balance, glycogen storage, ion transport, enzymes, etc. Gene Mutation and Genetics Part→ Focus on the autosomal recessive mutation and explain: How gene mutation affects the cell. How autosomal inheritance works. Compare the normal and mutated gene sequences simply. → Talk about possible consequences of a faulty hydrolytic enzyme.arrow_forwardCan you fill out those termsarrow_forward
- Explain down bellow what happens to the cell: Decreased pH in mitochondria Increased ATP Decreased pH in cytosol Increased hydrolysis Decreasing glycogen and triglycerides Increased MAP kinase activity Poor ion transport → For each one:→ What normally happens?→ What is wrong now?→ How does it mess up the cell?arrow_forwardAn 1100 pound equine patient was given 20 mg/kg sucralfate 3 times a day, 2.8 mg/kg famotidine twice a day, and 10mg/kg doxycycline twice a day. Sucralfate comes as a 1 gm tablet, famotidine as 20 mg tablets, and doxycycline as 100mg tablets. All are in bottles of 100 tablets.How many total mg are needed for the patient and how many tablets of each would be needed to provide each dose?How many bottles of each would be needed to have available if this patient were to be on this drug regimen for 5 days?arrow_forwardThe patient needs a solution of 2.5% dextrose in Lactated Ringer’s solution to run at 75 ml/hr for at least the next 12hours. LRS comes in fluid bags of 500 ml, 1 Liter, 3 Liters and 5 Liters. How can a 2.5% solution be made by adding50% dextrose to the LRS?arrow_forward
- “Gretchen” was a 68-pound canine who came to the VMTH as small animal surgery patient. She receivedacepromazine, 0.2 mg/kg from a 10 mg/ml solution and oxymorphone, 0.08 mg/kg from a 1 mg/ml solution before surgery.What are the mechanisms of action of acepromazine and oxymorphone? Why would they be given together?How many mg provide each dose and how many ml of each of these solutions were given?arrow_forwardAfter surgery, “Gretchen” was put on carprofen, 1 mg/pound bid (twice a day). The tablets come in 25, 75 and 100 mgsizes. Which size tablet would be appropriate?What is the mechanism of action of carprofen?An outpatient prescription was written for her so she would have enough for 10 days. How many tablets did she need?What information needs to be on her out-patient prescription?arrow_forwardJoden Koepp olor in chickens is due to incomplete dominance. BB = Black chicken, WW = White BLOOD TYPES Arhite chicken is In humans, Rh positive blood is dominant (R) over Rh negative blood (r). A man with type 0, Rh positive blood (whose mother had Rh negative blood), marries a woman with type AB, Rh negative blood. Several children were born. is? R R Genotypes Phenotypes RRR RR Rr Rr 4/16 RR R RR RK Rr Rr 4/16 rr 3/4 Rh posi 1/4 Rh negu 1/2 Rr rr rr rrrr 88 888 75 e genotype of the man? the genotype of the woman? The mother of the man had type AB blood.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Principles Of Radiographic Imaging: An Art And A ...Health & NutritionISBN:9781337711067Author:Richard R. Carlton, Arlene M. Adler, Vesna BalacPublisher:Cengage Learning
- Essentials of Pharmacology for Health ProfessionsNursingISBN:9781305441620Author:WOODROWPublisher:CengageCase Studies In Health Information ManagementBiologyISBN:9781337676908Author:SCHNERINGPublisher:Cengage
Principles Of Radiographic Imaging: An Art And A ...
Health & Nutrition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Cengage Learning
Essentials of Pharmacology for Health Professions
Nursing
ISBN:9781305441620
Author:WOODROW
Publisher:Cengage
Case Studies In Health Information Management
Biology
ISBN:9781337676908
Author:SCHNERING
Publisher:Cengage
DNA vs RNA (Updated); Author: Amoeba Sisters;https://www.youtube.com/watch?v=JQByjprj_mA;License: Standard youtube license