Concept explainers
Videos
What was the antiserum used in this experiment?
To fill:
Patient's serum
Introduction:
Agglutination is the phenomenon by which the antibody gets clumped to the antigenic components like flagellum, cell walls or capsules that are found on the cells of the bacterium. An unknown bacterium is usually mixed in saline and taken in a slide and antiserum containing known antibodies is added. Agglutination happens if the antibodies encounter antigens of the same species or strains as that of the antibody.
Answer to Problem 1Q
The patients serum is the antiserum used in this experiment.
Explanation of Solution
In the experiment, initially patient's serum is mixed with saline and wells are labeled and then dilutions of the antiserum( patient's serum) is made .Then to it antigen was added .The endpoint or the titer is the highest dilution in which clumping has taken place clearly. The patient's serum contains antibodies to the Salmonella entericaTyphimurium if clumping is detected. If there is no clumping or agglutination ,then there are no antibodies to the above mentioned bacterium. This forms the basis for Widal test or test for diagnosis of Typhoid fever.
Want to see more full solutions like this?
Chapter 43 Solutions
Laboratory Experiments in Microbiology (11th Edition)
- Selection of Traits What adaptations do scavengers have for locating and feeding on prey? What adaptations do predators have for capturing and consuming prey?arrow_forwardCompetition Between Species What natural processes limit populations from growing too large? What are some resources organisms can compete over in their natural habitat?arrow_forwardSpecies Interactions Explain how predators, prey and scavengers interact. Explain whether predators and scavengers are necessary or beneficial for an ecosystem.arrow_forward
- magine that you are conducting research on fruit type and seed dispersal. You submitted a paper to a peer-reviewed journal that addresses the factors that impact fruit type and seed dispersal mechanisms in plants of Central America. The editor of the journal communicates that your paper may be published if you make ‘minor revisions’ to the document. Describe two characteristics that you would expect in seeds that are dispersed by the wind. Contrast this with what you would expect for seeds that are gathered, buried or eaten by animals, and explain why they are different. (Editor’s note: Providing this information in your discussion will help readers to consider the significance of the research).arrow_forwardWhat is the difference between Uniporters, Symporters and Antiporters? Which of these are examples of active transport?arrow_forwardWhat are coupled transporters?arrow_forward
- How do histamine and prostaglandins help in the mobilization of leukocytes to an injury site? What are chemotactic factors? How do they affect inflammation process?arrow_forwardCompare and contrast neutrophils and macrophages. Describe two ways they are different and two ways they are similar.arrow_forwardDescribe the effects of three cytokines (not involved in the initial inflammation response). What cells release them?arrow_forward
Principles Of Radiographic Imaging: An Art And A ...Health & NutritionISBN:9781337711067Author:Richard R. Carlton, Arlene M. Adler, Vesna BalacPublisher:Cengage Learning
- Essentials of Pharmacology for Health ProfessionsNursingISBN:9781305441620Author:WOODROWPublisher:Cengage