Loose Leaf For Anatomy & Physiology: An Integrative Approach
3rd Edition
ISBN: 9781260162493
Author: McKinley Dr., Michael; O'Loughlin, Valerie; Bidle, Theresa
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 4.3, Problem 13WDL
Engulfing of a bacterium by a white blood cell occurs by what type of cellular transport?
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 4 Solutions
Loose Leaf For Anatomy & Physiology: An Integrative Approach
Ch. 4.1 - Prob. 1LOCh. 4.1 - What is the advantage of using a TEM instead of an...Ch. 4.1 - Prob. 2LOCh. 4.1 - Prob. 3LOCh. 4.1 - Prob. 2WDLCh. 4.1 - Prob. 4LOCh. 4.1 - Prob. 5LOCh. 4.1 - Prob. 6LOCh. 4.1 - Prob. 7LOCh. 4.1 - What are the three main structural features of a...
Ch. 4.1 - What cellular structure is responsible for forming...Ch. 4.2 - Prob. 8LOCh. 4.2 - How do lipids maintain the basic physical barrier...Ch. 4.2 - Prob. 9LOCh. 4.2 - Prob. 10LOCh. 4.2 - What type of plasma membrane protein provides the...Ch. 4.3 - Prob. 11LOCh. 4.3 - Prob. 12LOCh. 4.3 - How does O2 diffuse into a cell and CO2 diffuse...Ch. 4.3 - Prob. 8WDLCh. 4.3 - Prob. 13LOCh. 4.3 - LEARNING OBJECTIVE
14. Define osmotic pressure.
Ch. 4.3 - Prob. 15LOCh. 4.3 - WHAT DO YOU THINK?
1 Which setup would exhibit...Ch. 4.3 - Define osmosis.Ch. 4.3 - What occurs to the tonicity of a cell when it is...Ch. 4.3 - What general conclusion can you make concerning...Ch. 4.3 - Prob. 16LOCh. 4.3 - Prob. 17LOCh. 4.3 - Prob. 18LOCh. 4.3 - What transport process involved in the movement of...Ch. 4.3 - Engulfing of a bacterium by a white blood cell...Ch. 4.4 - Prob. 19LOCh. 4.4 - Prob. 20LOCh. 4.4 - Define a resting membrane potential.Ch. 4.4 - LEARNING OBJECTIVE
21. Explain the role of both...Ch. 4.4 - Prob. 22LOCh. 4.4 - Explain how the resting membrane potential is...Ch. 4.4 - Prob. 23LOCh. 4.5 - What are some examples of how cells communicate...Ch. 4.5 - Prob. 24LOCh. 4.5 - How do action of enzymatic receptors and G...Ch. 4.6 - Prob. 25LOCh. 4.6 - Prob. 26LOCh. 4.6 - Prob. 2WDTCh. 4.6 - Describe the general structure of both the...Ch. 4.6 - Prob. 19WDLCh. 4.6 - Prob. 27LOCh. 4.6 - Prob. 28LOCh. 4.6 - Which non-membrane-bound organelle functions (a)...Ch. 4.6 - Prob. 29LOCh. 4.6 - Prob. 30LOCh. 4.6 - Which cellular surface structure functions in (a)...Ch. 4.6 - Prob. 31LOCh. 4.6 - Which cellular junction (a) provides resistance to...Ch. 4.7 - Prob. 32LOCh. 4.7 - Prob. 33LOCh. 4.7 - What is the function of nuclear pores within the...Ch. 4.7 - What is the function of the nucleolus?Ch. 4.7 - Prob. 34LOCh. 4.7 - Describe the structural relationship of DNA and...Ch. 4.8 - Prob. 35LOCh. 4.8 - Prob. 36LOCh. 4.8 - What are the three major structures required for...Ch. 4.8 - Prob. 37LOCh. 4.8 - Prob. 38LOCh. 4.8 - Prob. 39LOCh. 4.8 - Prob. 3WDTCh. 4.8 - What is a codon and an anticodon?Ch. 4.8 - How is mRNA attached to ribosomes and translated...Ch. 4.8 - Prob. 40LOCh. 4.8 - Prob. 29WDLCh. 4.9 - LEARNING OBJECTIVE
41. Explain the structure and...Ch. 4.9 - Prob. 42LOCh. 4.9 - How is chromatin distinguished from a chromosome?Ch. 4.9 - Prob. 43LOCh. 4.9 - Prob. 44LOCh. 4.9 - Prob. 45LOCh. 4.9 - Prob. 4WDTCh. 4.9 - Describe the process of DNA replication that...Ch. 4.9 - What are the events that occur during the mitotic...Ch. 4.10 - Prob. 46LOCh. 4.10 - Prob. 47LOCh. 4.10 - What are the specific changes that occur to DNA...Ch. 4 - All of the following general functions are carried...Ch. 4 - _____ 2. The molecule that is responsible for most...Ch. 4 - Do You Know the Basics?
3. Which process does not...Ch. 4 - Prob. 4DYBCh. 4 - Prob. 5DYBCh. 4 - Prob. 6DYBCh. 4 - Prob. 7DYBCh. 4 - Prob. 8DYBCh. 4 - _____ 9. During this stage of mitosis, the...Ch. 4 - _____ 10. Erythrocytes do not have a nucleus. In...Ch. 4 - Prob. 11DYBCh. 4 - Prob. 12DYBCh. 4 - Describe the passive processes of membrane...Ch. 4 - Describe the active processes of membrane...Ch. 4 - List the membrane-bound structures, and describe...Ch. 4 - Prob. 16DYBCh. 4 - Prob. 17DYBCh. 4 - Prob. 18DYBCh. 4 - Prob. 19DYBCh. 4 - Explain the processes that occur in the different...Ch. 4 - Michael was born with Tay-Sachs disease. Which of...Ch. 4 - Prob. 2CALCh. 4 - Prob. 3CALCh. 4 - Prob. 4CALCh. 4 - Prob. 5CALCh. 4 - Prob. 1CSLCh. 4 - Prob. 2CSLCh. 4 - Prob. 3CSL
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