Loose Leaf For Anatomy & Physiology: An Integrative Approach
3rd Edition
ISBN: 9781260162493
Author: McKinley Dr., Michael; O'Loughlin, Valerie; Bidle, Theresa
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 4.6, Problem 21WDL
Which cellular surface structure functions in (a) increasing the cell’s surface area, and (b) movement of material past the cell?
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a) The amino acid sequence of the alpha chain terminates with a *. What does this symbol mean?
b) In your own words describe the function of the signal peptide and its final fate in post transcriptional modification.
c)How many amino acids form the precursor of insulin (preproinsulin)?
d) Since the C-peptide is cut out of proinsulin to create the final mature insulin (B-chain and A-chain) what role do you think the C-peptide might play in the biosynthesis of the mature insulin protein?
a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
Chapter 4 Solutions
Loose Leaf For Anatomy & Physiology: An Integrative Approach
Ch. 4.1 - Prob. 1LOCh. 4.1 - What is the advantage of using a TEM instead of an...Ch. 4.1 - Prob. 2LOCh. 4.1 - Prob. 3LOCh. 4.1 - Prob. 2WDLCh. 4.1 - Prob. 4LOCh. 4.1 - Prob. 5LOCh. 4.1 - Prob. 6LOCh. 4.1 - Prob. 7LOCh. 4.1 - What are the three main structural features of a...
Ch. 4.1 - What cellular structure is responsible for forming...Ch. 4.2 - Prob. 8LOCh. 4.2 - How do lipids maintain the basic physical barrier...Ch. 4.2 - Prob. 9LOCh. 4.2 - Prob. 10LOCh. 4.2 - What type of plasma membrane protein provides the...Ch. 4.3 - Prob. 11LOCh. 4.3 - Prob. 12LOCh. 4.3 - How does O2 diffuse into a cell and CO2 diffuse...Ch. 4.3 - Prob. 8WDLCh. 4.3 - Prob. 13LOCh. 4.3 - LEARNING OBJECTIVE
14. Define osmotic pressure.
Ch. 4.3 - Prob. 15LOCh. 4.3 - WHAT DO YOU THINK?
1 Which setup would exhibit...Ch. 4.3 - Define osmosis.Ch. 4.3 - What occurs to the tonicity of a cell when it is...Ch. 4.3 - What general conclusion can you make concerning...Ch. 4.3 - Prob. 16LOCh. 4.3 - Prob. 17LOCh. 4.3 - Prob. 18LOCh. 4.3 - What transport process involved in the movement of...Ch. 4.3 - Engulfing of a bacterium by a white blood cell...Ch. 4.4 - Prob. 19LOCh. 4.4 - Prob. 20LOCh. 4.4 - Define a resting membrane potential.Ch. 4.4 - LEARNING OBJECTIVE
21. Explain the role of both...Ch. 4.4 - Prob. 22LOCh. 4.4 - Explain how the resting membrane potential is...Ch. 4.4 - Prob. 23LOCh. 4.5 - What are some examples of how cells communicate...Ch. 4.5 - Prob. 24LOCh. 4.5 - How do action of enzymatic receptors and G...Ch. 4.6 - Prob. 25LOCh. 4.6 - Prob. 26LOCh. 4.6 - Prob. 2WDTCh. 4.6 - Describe the general structure of both the...Ch. 4.6 - Prob. 19WDLCh. 4.6 - Prob. 27LOCh. 4.6 - Prob. 28LOCh. 4.6 - Which non-membrane-bound organelle functions (a)...Ch. 4.6 - Prob. 29LOCh. 4.6 - Prob. 30LOCh. 4.6 - Which cellular surface structure functions in (a)...Ch. 4.6 - Prob. 31LOCh. 4.6 - Which cellular junction (a) provides resistance to...Ch. 4.7 - Prob. 32LOCh. 4.7 - Prob. 33LOCh. 4.7 - What is the function of nuclear pores within the...Ch. 4.7 - What is the function of the nucleolus?Ch. 4.7 - Prob. 34LOCh. 4.7 - Describe the structural relationship of DNA and...Ch. 4.8 - Prob. 35LOCh. 4.8 - Prob. 36LOCh. 4.8 - What are the three major structures required for...Ch. 4.8 - Prob. 37LOCh. 4.8 - Prob. 38LOCh. 4.8 - Prob. 39LOCh. 4.8 - Prob. 3WDTCh. 4.8 - What is a codon and an anticodon?Ch. 4.8 - How is mRNA attached to ribosomes and translated...Ch. 4.8 - Prob. 40LOCh. 4.8 - Prob. 29WDLCh. 4.9 - LEARNING OBJECTIVE
41. Explain the structure and...Ch. 4.9 - Prob. 42LOCh. 4.9 - How is chromatin distinguished from a chromosome?Ch. 4.9 - Prob. 43LOCh. 4.9 - Prob. 44LOCh. 4.9 - Prob. 45LOCh. 4.9 - Prob. 4WDTCh. 4.9 - Describe the process of DNA replication that...Ch. 4.9 - What are the events that occur during the mitotic...Ch. 4.10 - Prob. 46LOCh. 4.10 - Prob. 47LOCh. 4.10 - What are the specific changes that occur to DNA...Ch. 4 - All of the following general functions are carried...Ch. 4 - _____ 2. The molecule that is responsible for most...Ch. 4 - Do You Know the Basics?
3. Which process does not...Ch. 4 - Prob. 4DYBCh. 4 - Prob. 5DYBCh. 4 - Prob. 6DYBCh. 4 - Prob. 7DYBCh. 4 - Prob. 8DYBCh. 4 - _____ 9. During this stage of mitosis, the...Ch. 4 - _____ 10. Erythrocytes do not have a nucleus. In...Ch. 4 - Prob. 11DYBCh. 4 - Prob. 12DYBCh. 4 - Describe the passive processes of membrane...Ch. 4 - Describe the active processes of membrane...Ch. 4 - List the membrane-bound structures, and describe...Ch. 4 - Prob. 16DYBCh. 4 - Prob. 17DYBCh. 4 - Prob. 18DYBCh. 4 - Prob. 19DYBCh. 4 - Explain the processes that occur in the different...Ch. 4 - Michael was born with Tay-Sachs disease. Which of...Ch. 4 - Prob. 2CALCh. 4 - Prob. 3CALCh. 4 - Prob. 4CALCh. 4 - Prob. 5CALCh. 4 - Prob. 1CSLCh. 4 - Prob. 2CSLCh. 4 - Prob. 3CSL
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- 1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forward
- Based on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have shipped your samples off for sequencing and are now waiting for the results. Out of curiosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidase enzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you do see a color change to purple during the Oxidase test. What results can you conclude from this? O Catalase-/ Oxidase + O Catalase +/ Oxidase + Catalase + / Oxidase- O Catalase / Oxidase - O None of the abovearrow_forward
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forward
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