Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 42.4, Problem 5RIA
Summary Introduction
Metagenomics (term coined in 1998) is the study of genetic material (genome) of microbial populations obtained in the environment. Starting with a mixture of DNA, metagenomics library screening aims to identify novel genetic components that have beneficial functions. These screenings are conducted by two approaches: genetic screens where known groups of genes are examined to identify novel functions and functional screens where
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Chapter 42 Solutions
Prescott's Microbiology
Ch. 42.1 - Why are the cells first fed lactose and then...Ch. 42.1 - What critical limiting factors are used in the...Ch. 42.1 - Prob. 2RIACh. 42.1 - Discuss the major uses for biopolymers and...Ch. 42.1 - What kinds of molecular changes result from...Ch. 42.1 - Prob. 5RIACh. 42.2 - Why is there interest in developing alternatives...Ch. 42.2 - What types of microbes are good candidates for the...Ch. 42.3 - What is the objective of the scale-up process and...Ch. 42.3 - What parameters can be monitored in a modern,...
Ch. 42.3 - Which of the following are secondary metabolites:...Ch. 42.4 - Why are modular genes particularly suitable as...Ch. 42.4 - Prob. 2MICh. 42.4 - Prob. 1RIACh. 42.4 - Prob. 2RIACh. 42.4 - What is metabolic engineering? Besides...Ch. 42.4 - What is high-throughput screening and why has it...Ch. 42.4 - Prob. 5RIACh. 42.5 - What is the Ti plasmid and how is it modified for...Ch. 42.5 - How is Bt toxin produced and why is it so widely...Ch. 42.5 - Can you think of other traits that might be...Ch. 42.6 - MICRO INQUIRY Why is it advantageous to have the...Ch. 42.6 - Why are diatoms and magnetotactic bacteria of...Ch. 42.6 - Prob. 2RIACh. 42 - Prob. 1CHICh. 42 - Prob. 2CHICh. 42 - The detection of unexploded land mines is a...
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- A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation? The cell membranes are more resistant than normal. An isotonic solution had been added instead of water. A solution of 0.1 M NaCl had been added instead of water. Not enough water had been added to the red blood cell pellet. The man had sickle-cell anaemia.arrow_forwardWith reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobinarrow_forwardWith reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobinarrow_forward
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