Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 42, Problem 3CHI
The detection of unexploded land mines is a horrific problem. Unexploded ordinances emit a small amount of nitroaromatic compounds, affording the opportunity to develop a biosensor. A Pseudomonas putida strain has been constructed with a mutated toluene responsive regulator. When activated by bi- and trinitro-substituted toluenes, the regulator triggers the expression of genes transcriptionally fused to GFP (see figure 17.15). When spread on soil spotted with nitrotoluenes, there is sufficient activation of GFP-generated light that the location of the chemicals is evident.
Draw a flow chart of how the signal is emitted, received, and transduced into a light signal that would enable you to explain this biosensor to military personnel.
Read the original paper: Garmendia, J., et al. 2008. Tracing explosives in soil with transcriptional regulators for Pseudomonas putida evolved for responding to nitrotoluenes. Microbial Biotechnol. 1:236.
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Chapter 42 Solutions
Prescott's Microbiology
Ch. 42.1 - Why are the cells first fed lactose and then...Ch. 42.1 - What critical limiting factors are used in the...Ch. 42.1 - Prob. 2RIACh. 42.1 - Discuss the major uses for biopolymers and...Ch. 42.1 - What kinds of molecular changes result from...Ch. 42.1 - Prob. 5RIACh. 42.2 - Why is there interest in developing alternatives...Ch. 42.2 - What types of microbes are good candidates for the...Ch. 42.3 - What is the objective of the scale-up process and...Ch. 42.3 - What parameters can be monitored in a modern,...
Ch. 42.3 - Which of the following are secondary metabolites:...Ch. 42.4 - Why are modular genes particularly suitable as...Ch. 42.4 - Prob. 2MICh. 42.4 - Prob. 1RIACh. 42.4 - Prob. 2RIACh. 42.4 - What is metabolic engineering? Besides...Ch. 42.4 - What is high-throughput screening and why has it...Ch. 42.4 - Prob. 5RIACh. 42.5 - What is the Ti plasmid and how is it modified for...Ch. 42.5 - How is Bt toxin produced and why is it so widely...Ch. 42.5 - Can you think of other traits that might be...Ch. 42.6 - MICRO INQUIRY Why is it advantageous to have the...Ch. 42.6 - Why are diatoms and magnetotactic bacteria of...Ch. 42.6 - Prob. 2RIACh. 42 - Prob. 1CHICh. 42 - Prob. 2CHICh. 42 - The detection of unexploded land mines is a...
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