BIOLOGY(LL)-W/ACCESS CODE >CUSTOM<
12th Edition
ISBN: 9781264058167
Author: Raven
Publisher: MCG CUSTOM
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Chapter 42, Problem 3A
Summary Introduction
Introduction:
Botox is basically a derivative of Botulinum toxin that is nowadays used for cosmetic purposes. Botox injections are widely being used to treat wrinkles in the adults. Botulinum toxin produced by Clostridium botulinum, is basically known to cause food poisoning, but it has another function to inhibit neurotransmitter acetylcholine at the neuromuscular junction.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 42 Solutions
BIOLOGY(LL)-W/ACCESS CODE >CUSTOM<
Ch. 42.1 - Differentiate between subdivisions of the...Ch. 42.1 - Prob. 2LOCh. 42.1 - Explain the roles of the different nervous system...Ch. 42.2 - Contrast the relative concentrations of important...Ch. 42.2 - Prob. 2LOCh. 42.2 - Prob. 3LOCh. 42.3 - Prob. 1LOCh. 42.3 - Prob. 2LOCh. 42.3 - Prob. 3LOCh. 42.4 - Prob. 1LO
Ch. 42.4 - Prob. 2LOCh. 42.4 - Prob. 3LOCh. 42.5 - Describe the organization of the peripheral...Ch. 42.5 - Prob. 2LOCh. 42.5 - Prob. 3LOCh. 42.5 - Prob. 4LOCh. 42 - Data analysis Draw the resulting potentials for...Ch. 42 - Prob. 2DACh. 42 - Which of the following best describes the...Ch. 42 - The ____ cannot be controlled by conscious...Ch. 42 - Prob. 3UCh. 42 - Inhibitory neurotransmitters a. hyperpolarize...Ch. 42 - White matter is ______, and gray matter is...Ch. 42 - During an action potential a. the rising phase is...Ch. 42 - Prob. 7UCh. 42 - Imagine that you are doing an experiment on the...Ch. 42 - The Na+/K+ ATPase pump is a. not required for...Ch. 42 - Prob. 3ACh. 42 - The following is a list of the components of a...Ch. 42 - Prob. 5ACh. 42 - As you sit quietly reading this sentence, the part...Ch. 42 - G proteincoupled receptors are involved in the...Ch. 42 - Tetraethylammonium (TEA) is a drug that blocks...Ch. 42 - Describe the status of the Na+ and K+ channels at...Ch. 42 - Describe the steps required to produce an...Ch. 42 - Prob. 4S
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