Biology: The Unity and Diversity of Life (MindTap Course List)
15th Edition
ISBN: 9781337408332
Author: Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher: Cengage Learning
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Chapter 40, Problem 2DAA
Pesticide Residues in Urine To carry the USDA’s organic label (right), food must be produced without synthetic pesticides that farmers often use on conventionally grown, fruits, vegetables, and many grains.
Chensheng Lu of Emory University used urine testing to see whether eating organic food has a significant effect on the levels of pesticides in children's bodies (FIGURE 40.11). Over the course of 15 days, Lu and his colleagues collected the urine of 23 children (aged 3 to 11) and tested it for breakdown products of two synthetic pesticides. The children ate their normal diet of conventionally grown foods for three days, switched to organic versions of the same foods and drinks for five days, then returned to their convention.al diet for a week
FIGURE 40.11 Concentrations of metabolites of two pesticides (malathion and chlorpyrifos) in children’s urine during the three different phases of the study. The difference in the mean level of metabolites between the organic and conventional phases of the study was statistically significant.
During which phase of the experiment was the maximum level of the chlorpyrifos metabolite detected?
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 40 Solutions
Biology: The Unity and Diversity of Life (MindTap Course List)
Ch. 40 - Pesticide Residues in Urine To carry the USDAs...Ch. 40 - Pesticide Residues in Urine To carry the USDAs...Ch. 40 - Prob. 3DAACh. 40 - An insect's ___ deliver nitrogen-rich waste to its...Ch. 40 - Bowman's capsule, the start of the tubular part of...Ch. 40 - Fluid that enters Bowman's capsule flows directly...Ch. 40 - Blood pressure forces water and small solutes into...Ch. 40 - Kidneys return most of the water and small solutes...Ch. 40 - Prob. 6SQCh. 40 - Prob. 7SQ
Ch. 40 - Match each .structure with a function. _____...Ch. 40 - Prob. 9SQCh. 40 - Prob. 10SQCh. 40 - Prob. 11SQCh. 40 - Prob. 12SQCh. 40 - Prob. 13SQCh. 40 - Prob. 14SQCh. 40 - Prob. 15SQCh. 40 - Like desert rodents such as the kangaroo rat,...Ch. 40 - Compared to closely related species that live in...Ch. 40 - Prob. 3CTCh. 40 - Drinking alcohol inhibits ADH secretion. What...
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