Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
6th Edition
ISBN: 9780815344322
Author: Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher: W. W. Norton & Company
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Chapter 4, Problem 6P
Summary Introduction

To explain:  The nucleotide composition % obtained from the bacterial virus M13 is peculiar.

Concept introduction: Purines and pyrimidines constitute the nucleotides of the DNA. Adenine and guanine are the purines. Cytosine and thymine are the pyrimidines. In a double-stranded DNA, adenine always bond with thymine and guanine always bond with cytosine. Chargaff’s rule states that in a double-stranded DNA, the number of purines is always equal to the number of pyrimidines that is the ratio of purines to pyrimidines should always be 1:1. Also the amount of adenine must be equal to the amount of thymine, and amount of guanine must be equal to the amount of thymine based on the purine and pyrimidine pairing rule.

Summary Introduction

To explain:  The reason why the results are peculiar.

Concept introduction: Purines and pyrimidines constitute the nucleotides of the DNA. Adenine and guanine are the purines. Cytosine and thymine are the pyrimidines. In a double-stranded DNA, adenine always bond with thymine and guanine always bond with cytosine. Chargaff’s rule states that in a double-stranded DNA, the number of purines is always equal to the number of pyrimidines that is the ratio of purines to pyrimidines should always be 1:1. Also the amount of adenine must be equal to the amount of thymine, and amount of guanine must be equal to the amount of thymine based on the purine and pyrimidine pairing rule.

Summary Introduction

To explain:  The reasons why the values vary largely.

Concept introduction: Purines and pyrimidines constitute the nucleotides of the DNA. Adenine and guanine are the purines. Cytosine and thymine are the pyrimidines. In a double-stranded DNA, adenine always bond with thymine and guanine always bond with cytosine. Chargaff’s rule states that in a double-stranded DNA, the number of purines is always equal to the number of pyrimidines that is the ratio of purines to pyrimidines should always be 1:1. Also the amount of adenine must be equal to the amount of thymine, and amount of guanine must be equal to the amount of thymine based on the purine and pyrimidine pairing rule.

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