The reason why white areas have formed near the rim of the red colony. Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.

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Answer 1

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Chapter 4, Problem 13P

Summary Introduction

To explain: The reason why white areas have formed near the rim of the red colony.

Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.

Summary Introduction

To explain: The propagation of the transcriptional state of the Ade2 gene from mother to daughter cells.

Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.

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Amino Acid Coclow TABle 3' Gly Phe Leu (G) (F) (L) 3- Val (V) Arg (R) Ser (S) Ala (A) Lys (K) CAG G Glu Asp (E) (D) Ser (S) CCCAGUCAGUCAGUCAG 0204 C U A G C Asn (N) G 4 A AGU C GU (5) AC C UGA A G5 C CUGACUGACUGACUGAC Thr (T) Met (M) lle £€ (1) U 4 G Tyr Σε (Y) U Cys (C) C A G Trp (W) 3' U C A Leu בוט His Pro (P) ££ (H) Gin (Q) Arg 흐름 (R) (L) Start Stop 8. Transcription and Translation Practice: (Video 10-1 and 10-2) A. Below is the sense strand of a DNA gene. Using the sense strand, create the antisense DNA strand and label the 5' and 3' ends. B. Use the antisense strand that you create in part A as a template to create the mRNA transcript of the gene and label the 5' and 3' ends. C. Translate the mRNA you produced in part B into the polypeptide sequence making sure to follow all the rules of translation. 5'-AGCATGACTAATAGTTGTTGAGCTGTC-3' (sense strand) 4

Answer 2

Question

Chapter 4, Problem 13P

Summary Introduction

To explain: The reason why white areas have formed near the rim of the red colony.

Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.

Summary Introduction

To explain: The propagation of the transcriptional state of the Ade2 gene from mother to daughter cells.

Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.

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Answer 3

Question

Chapter 4, Problem 13P

Summary Introduction

To explain: The reason why white areas have formed near the rim of the red colony.

Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.

Summary Introduction

To explain: The propagation of the transcriptional state of the Ade2 gene from mother to daughter cells.

Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.

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Answer 4

Question

Chapter 4, Problem 13P

Summary Introduction

To explain: The reason why white areas have formed near the rim of the red colony.

Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.

Summary Introduction

To explain: The propagation of the transcriptional state of the Ade2 gene from mother to daughter cells.

Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.

Expert Solution & Answer

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Answer 5

Chapter 4, Problem 13P

Summary Introduction

To explain: The reason why white areas have formed near the rim of the red colony.

Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.

Summary Introduction

To explain: The propagation of the transcriptional state of the Ade2 gene from mother to daughter cells.

Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.

Answer 6

Chapter 4, Problem 13P

Summary Introduction

To explain: The reason why white areas have formed near the rim of the red colony.

Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.

Answer 7

Chapter 4, Problem 13P

Summary Introduction

To explain: The reason why white areas have formed near the rim of the red colony.

Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.

Answer 8

Summary Introduction

To explain: The propagation of the transcriptional state of the Ade2 gene from mother to daughter cells.

Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.

Answer 9

Summary Introduction

To explain: The propagation of the transcriptional state of the Ade2 gene from mother to daughter cells.

Concept introduction: Position effect variegation also known as gene juxtaposition is a phenomenon where a gene which is normally present in the transcriptionally active (euchromatin) region was transposition to a transcriptionally inactive region (heterochromatin). This leads to loss of activity of the gene. The yeast Ade2 encodes one of the enzymes that required for the adenine biosynthesis. The gene expression is repressed in the presence of adenine and is slightly increased in the amino acid starvation conditions. When the gene is expressed then the yeast colony will be in white and when the gene is not expressed then the colony will have red pigment.

Answer 10

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Answer 13

Ch. 4 - Prob. 1P Ch. 4 - Prob. 2P Ch. 4 - Prob. 3P Ch. 4 - Prob. 4P Ch. 4 - Prob. 5P Ch. 4 - Prob. 6P Ch. 4 - Prob. 7P Ch. 4 - Prob. 8P Ch. 4 - Prob. 9P Ch. 4 - Prob. 10P

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