Microbiology with Diseases by Body System (5th Edition)
5th Edition
ISBN: 9780134477206
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 4, Problem 5CT
Summary Introduction
To answer:
To find the resolution of two objects at 400nm and 40nm apart by using 100× oil lens.
Introduction:
A microscope is a device used to view the magnified image of an object. There are two types of lens used in the microscope namely, the objective and the ocular lens. The ocular lens has 10× power and the objective lenses have 10×, 40×, and 100× power respectively.
The resolving power of a microscope was increased by using the immersion oil. It was attained by immersing the objective lens and the slide containing the sample in a transparent oil. The 100× objective lens is used for the oil immersion procedure.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Please explain the table
3D dimensionality is a limitation of the compound
microscope. Depth of field, DOF, describes dimensionality
form top to bottom and can be observed with colored cross
threads. Observe the crossed thread slide on low power
(4x), then on medium power (10x), then on high power
(40x objective magnification).
Which crossed fiber is on top?
How do you know?
Using a good compound light microscope with a resolving of 0.3 micro meter a 10x ocular lens ,and a 100 x oil immersion lens, would you be able to discern two objects separated by 0.4 micro meter?0.2 micro meter? 300 neno meter ? Explain your reasoning.
Chapter 4 Solutions
Microbiology with Diseases by Body System (5th Edition)
Ch. 4 - Prob. 1TMWCh. 4 - Why is a Gram-negative bacterium colorless but a...Ch. 4 - Why didnt Linnaeus create taxonomic groups for...Ch. 4 - Necrotizing Fasciitis Fever, chills, nausea,...Ch. 4 - Why is magnification high and color absent in an...Ch. 4 - Prob. 1MCCh. 4 - Prob. 2MCCh. 4 - Prob. 3MCCh. 4 - Curved glass lenses _________ light. a. refract b....Ch. 4 - Prob. 5MC
Ch. 4 - Prob. 6MCCh. 4 - Prob. 7MCCh. 4 - Prob. 8MCCh. 4 - Prob. 9MCCh. 4 - In the binomial system at nomenclature, which term...Ch. 4 - Prob. 1FIBCh. 4 - Prob. 2FIBCh. 4 - Prob. 3FIBCh. 4 - Fill in the Blanks 4. ___________ refers to...Ch. 4 - Fill in the Blanks 5. Cationic chromophores such...Ch. 4 - Prob. 1VICh. 4 - Label the microscope.Ch. 4 - Prob. 1SACh. 4 - Critique the following definition of magnification...Ch. 4 - Prob. 3SACh. 4 - Put the following substances in the order they are...Ch. 4 - Prob. 5SACh. 4 - Prob. 6SACh. 4 - Prob. 7SACh. 4 - Prob. 8SACh. 4 - Miki came home from microbiology lab with green...Ch. 4 - Why is the definition of species as successfully...Ch. 4 - With the exception of the discovery of new...Ch. 4 - Prob. 4CTCh. 4 - Prob. 5CTCh. 4 - In what ways are the Gram stain and the acid-fast...Ch. 4 - Microbiologists have announced the discovery of...Ch. 4 - Why is the genus name Coccus placed within...Ch. 4 - A clinician obtains a specimen of urine from a...Ch. 4 - Using the following terms, fill in the following...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- If the total magnification of a microscope is 700X and the ocular lens magnifies by a factor of 10x, what is the magnification of the objective lens that was used? Paragraph BIUarrow_forwardExplain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?arrow_forwardD) When performing fluorescence microscopy what are the stokes shift and why is it better to have fluorochromes with a large stokes shift? E) What is photobleaching and what is done when imaging histological samples to overcome it when performing fluorescence microscopy?arrow_forward
- What’s the aim of using a microscope for examining? What can the kohler illumination technique tell us? What are the sources of error that can impact the data ?arrow_forwardPrevious question: For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope. - The Answer I got for this question was 3/4mm.arrow_forwardIf the ocular lens of a microscope has 10X magnifying power, and the 40X objective lens is being used for observation, what is the total magnification for the viewed image? 10x 43x 1000x 400xarrow_forward
- Calculate the diameter of the field of view for each total magnification on your microscope in millimeters (mm) and then convert this value to micrometers (um): 4.5 mm = 4,500 um Scanning (40X): 1.8 mm x 100X/40X = Low power (100X): FOV diameter 1.8 mm = 1,800 um High power (400X): 1.8 mm x 100X/400X = 0.45 mm = 450 um Oil immersion (1000X): O.18m 180 um 1.8 mm x 100x/1000X = Draw and estimate the length of a single Euglena (high power) and Paramecium (low power): Paramecium Euglena total magnification total magnification 400x yoox FOV diam. FOV diam. um uni length length umarrow_forwardYou look through a microscope and count 8 cells that fit across the diameter of your field of view with the 40X lens. Knowing that your field diameter with the 4X lens is 5 mm, answer the following: a) What is the field diameter at 40X? b) What is the size of each cell you viewed, in mm? in um?arrow_forward1)You can see something at 10x but not at 40x or 100x, what do you do? 2)Your microscope has light, the slide looks like it's in the right place, but you can't fully focus on the smear. Do you move up to the next magnification? Explain why?arrow_forward
- Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope. c) After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred Provide a description of the procedure to prepare an acidic stain of bacteria using Nigrosin as it would appear in the Methods section.arrow_forwardHow many coccus cells (0.0005 mm in diameter) could fit across the entire FOV of the 100x objective lens (assuming the DFOV of the 100x lens is 180 μm?arrow_forwardImagine you are using a microscope with 5x eye pieces (instead of 10x like ours). Complete the sentence. If you change from the 10x objective lens to the 40x objective lens, the total magnification changes from [Select] ř to | Select ] Select] 15x 50x 10x 梦 $ 99+arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Principles Of Radiographic Imaging: An Art And A ...Health & NutritionISBN:9781337711067Author:Richard R. Carlton, Arlene M. Adler, Vesna BalacPublisher:Cengage Learning
Principles Of Radiographic Imaging: An Art And A ...
Health & Nutrition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Cengage Learning