Microbiology for Surgical Technologists
2nd Edition
ISBN: 9781337243209
Author: Margaret Rodriguez
Publisher: Cengage Learning US
expand_more
expand_more
format_list_bulleted
Question
Chapter 4, Problem 4UTM
Summary Introduction
To explain: The threat of bacteria which is used to test biological sterilizer.
Introduction:
The autoclave is a device that is used to sterilize the biological laboratory equipment. It uses the power of steam (high pressure and moist) to kill the microbes that are resistant to powerful detergents and boiling water.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Plastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures?
METHOD:RATIONALE:
The statement, “In the laboratory, a sterile inoculating loop is moved across the agar
surface
in a culture dish, thinning a sample and isolating individuals", describes which technique?
Why do you think rapid methods so helpful to identify pathogens microbes in clinical settings.
Why are traditional test such as metabolic test not always used to ID clinically important microbes
Please make it simple and short.Thanks
Chapter 4 Solutions
Microbiology for Surgical Technologists
Ch. 4 - We are a product of our genes, so they say;...Ch. 4 - We are a product of our genes, so they say;...Ch. 4 - We are a product of our genes, so they say;...Ch. 4 - Prob. 4TBPCh. 4 - A surgical technologist working in the Central...Ch. 4 - A surgical technologist working in the Central...Ch. 4 - A surgical technologist working in the Central...Ch. 4 - Prob. 4UTMCh. 4 - Prob. 5UTM
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which type of bacteria would be used to test the autoclave?arrow_forwardA surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. What must be done with the bacterial sample after it is processed in the sterilizer?arrow_forwardA surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which characteristic of the species used determines whether sterility was achieved when autoclaved?arrow_forward
- The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?arrow_forwardHow do eosin-methylene blue (EMB) agar plates work? What organism(s) are they designed to detect? How would a positive test appear on the plate?arrow_forwardA patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution series shown in the Figure ( Dilution.jpg) is prepared and 1 ml from each tube is plated. Plates were grown 24 hours resulting in colonies in each plate. Which plate you will use to quantify the bacteria in the patient sample? Why did you pick that plate? What is the dilution factor used in this series? Calculate the number of bacteria in the original patient sample. What would you do and why, if there were too many colonies to count on each plate?arrow_forward
- A class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/Larrow_forwardWhich one of the following microorganisms would you use as a quality control check to ensure that your sterile technique method is properly working? S. aureus, a gram-positive bacterium E. coli, a gram-negative bacterium B. cereus, an endospore-forming bacterium M. leprae, an acid-fast bacterium T. pallidum, a flagellated, disease causing bacteriumarrow_forwardBased on the found under #4 inoculating plates order, and the definitions above, what can you assume the order of plating cultures is based on? *arrow_forward
- You were instructed to add 1.0ml out of 4.0ml of an undiluted sample to 99ml of sterile diligent you add the entire 4.0ml to 99 ml. a) what was your intended dilution factor? b) what was your actual dilution factor?arrow_forwardThere are so many microbes in a single mL of culture, it is very difficult to perform one dilution to produce countable cells. Microbiologists need to perform a dilution series, where multiple dilutions are performed in sequence to arrive at the correct dilution. Dilutions are cumulative. Multiple the series of dilutions together to find the final dilution value. If 3 serial dilutions are performed, each with a value of 0.01, what is the cumulative dilution? Express your answer as an exponent, e.g. 0.1 would be 1e-1 and 0.01 would be 1e-2arrow_forwardTopic: Spread-Plate Method Agar was not completely set prior to inoculating with the organism. Effect:Rationale:arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Microbiology for Surgical Technologists (MindTap ...BiologyISBN:9781111306663Author:Margaret Rodriguez, Paul PricePublisher:Cengage LearningComprehensive Medical Assisting: Administrative a...NursingISBN:9781305964792Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy CorreaPublisher:Cengage Learning
Microbiology for Surgical Technologists (MindTap ...
Biology
ISBN:9781111306663
Author:Margaret Rodriguez, Paul Price
Publisher:Cengage Learning
Comprehensive Medical Assisting: Administrative a...
Nursing
ISBN:9781305964792
Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa
Publisher:Cengage Learning
Infection Prevention and Control; Author: thecityoftoronto;https://www.youtube.com/watch?v=jx9sRYmBW3Q;License: Standard Youtube License