Microbiology for Surgical Technologists
2nd Edition
ISBN: 9781337243209
Author: Margaret Rodriguez
Publisher: Cengage Learning US
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Chapter 4, Problem 3UTM
A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items.
What must be done with the bacterial sample after it is processed in the sterilizer?
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What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube
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c.
Inoculating needle
d.
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Chapter 4 Solutions
Microbiology for Surgical Technologists
Ch. 4 - We are a product of our genes, so they say;...Ch. 4 - We are a product of our genes, so they say;...Ch. 4 - We are a product of our genes, so they say;...Ch. 4 - Prob. 4TBPCh. 4 - A surgical technologist working in the Central...Ch. 4 - A surgical technologist working in the Central...Ch. 4 - A surgical technologist working in the Central...Ch. 4 - Prob. 4UTMCh. 4 - Prob. 5UTM
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- A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which type of bacteria would be used to test the autoclave?arrow_forwardHow many different types of colonies can you find on the blood agar plate? describe each. (As shown in pictures below) What possible types of bacteria ? (As shown in pictures below)arrow_forwardWhat advantage does the oil immersion objective have over the low power objective when doing bacterial examination?arrow_forward
- A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution series shown in the Figure ( Dilution.jpg) is prepared and 1 ml from each tube is plated. Plates were grown 24 hours resulting in colonies in each plate. Which plate you will use to quantify the bacteria in the patient sample? Why did you pick that plate? What is the dilution factor used in this series? Calculate the number of bacteria in the original patient sample. What would you do and why, if there were too many colonies to count on each plate?arrow_forwardA surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which characteristic of the species used determines whether sterility was achieved when autoclaved?arrow_forwardA student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 Celciusarrow_forward
- What is the purpose of flaming an inoculating loop? How will you know when you have flamed the loop or needle long enough? Why is it necessary to cool the inoculating loop prior to obtaining the bacterial sample? In which direction should you move the inoculating loop in the Bunsen burner flame? (i.e. from the handle to the loop or from the loop to the handle)arrow_forwardKousei works at a company that produces sweetened condensed milk. If one or more samples exceed 104 bacterial CFU/mL (or CFU/g) the entire lot produced will be rejected. Kousei's boss, Kaori, wanted to know if the lot passed the FDA guidelines. The following bacterial counts were obtained after serial dilution and pour plating of the samples: Type of Dilutions plated 10-4 10-³ 10'5 Colony Plate 1 Plate 2 Plate 1 Plate 2 Plate 3 Plate 1 Plate 2 Plate 3 Plate 3 244 Bacteria 288 251 99 81 75 24 21 15 Based on the bacterial counts obtained, will the lot be rejected? Yes, because the computed CFU/mL is 2.5 x 10^7 OYes, because the computed CFU/mL is 3.0 x 10^6 No, because the computed CFU/mL is 3.0 x 10^3 No, because the computed CFU/mL is 2.5 x 10^3 OYes, because the computed CFU/mL is 3.8 x 10^5 O No, because the computed CFU/mL is 3.0 x 10^3 O Information is lacking so CFU/mL cannot be computedarrow_forwardBased on the found under #4 inoculating plates order, and the definitions above, what can you assume the order of plating cultures is based on? *arrow_forward
- How do eosin-methylene blue (EMB) agar plates work? What organism(s) are they designed to detect? How would a positive test appear on the plate?arrow_forwardYou are given a mix culture of S. aureus,E. coli and P. aeruginosa. Besides the streak plate method what other methods could you use to separate the bacteria? Please state what method(s) you would use, the result you would be looking for to help identify each bacterium and the interpretation of the result.arrow_forwardWould you use an inoculating loop to transfer bacteria to an agar deep tube? Would you use an inoculating loop to transfer bacteria to an agar deep tube? Yes, as long as you insert the loop into the agar smoothly. No, a needle would be best since it will do the least amount of damage to the agar and allow it to reseal around the inserted bacteria. It depends on which bacteria you are transferring. Yes, as long as you are careful when streaking on the surface of the agar.arrow_forward
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