The relationship between the Burger's vector oreintation and the dislocation axis for edge dislocations and screw dislocations needs to be explained. Concept Introduction: The burgers vector is a vector in material science, and it is denoted by b representing the magnitude as well as direction of the distortion of lattice due to dislocation in a crystal lattice. On visualizing the crystal structure before the dislocation, the magnitude and direction of vector can be understood better. This is known as the perfect crystal structure. Depending on the plane of dislocation, the direction of the vector is decided, and the magnitude is calculated as follows: ‖ b ‖ = ( a 2 ) h 2 + k 2 + l 2 Here, a is edge length and h, k, and l are Burgers vector's components.

Question

7) You are tasked with separating two proteins by ion exchange chromatography on a 30 cm long column with an inner diameter of 2 cm. The resin has a diameter of 100 μm and a void fraction of 0.3, and your mobile phase flows through the column at a rate of Q = 5 cm³/min. The Van Deemter coefficients A, B, and C have been determined to be 0.0228 cm, 0.0036 cm²/min, and 0.00053 min, respectively, for both proteins. Protein A elutes from the column with an average retention time of 27 min and standard deviation of 0.8 min. Protein B elutes from the column. with an average retention time of 33.8 min and standard deviation of 1.0. a) How many theoretical plates does the column contain? b) What flow rate (Q) will give you the maximum resolution? c) What is the minimum height of a theoretical plate for the system?

Answer 1

Question

Chapter 4, Problem 4.21P

Interpretation Introduction

Interpretation:

The relationship between the Burger's vector oreintation and the dislocation axis for edge dislocations and screw dislocations needs to be explained.

Concept Introduction:

The burgers vector is a vector in material science, and it is denoted by b representing the magnitude as well as direction of the distortion of lattice due to dislocation in a crystal lattice. On visualizing the crystal structure before the dislocation, the magnitude and direction of vector can be understood better. This is known as the perfect crystal structure. Depending on the plane of dislocation, the direction of the vector is decided, and the magnitude is calculated as follows:

‖b‖=(a2)h2+k2+l2

Here, a is edge length and h, k, and l are Burgers vector's components.

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