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1) Indicate whether each statement below is True or False: a) In extractions, small proteins usually end up in the heavy phase. b) In extractions, large proteins usually end up in the heavy phase. c) Tie line length on a phase diagram has no effect on protein partitioning. d) For extraction, we assume that each stage reaches equilibrium. e) Isotherms that are concave down lead to self-sharpening solute fronts in adsorption. f) When scaling up an adsorption unit, the LUB scales proportionally with adsorber length. g) For chromatography, separation efficiency increases when HETP increases. h) In size exclusion chromatography, smaller molecules elute from the column first. i) In hydrophobic interaction chromatography, very hydrophobic proteins elute in water. j) Molecules at very high concentrations elute from the column as a perfect Gaussian peak. k) In chromatography scale-up, resolution will increase as column diameter increases. 1) A good adsorbent typically has no porosity. m) Polymer-based resins are usually more compressible than silica-based resins. n) Changing pH in an ion exchange chromatography process can alter protein binding. 으 o) Histidine residues will bind to immobilized Ni²+. 1

1) Indicate whether each statement below is True or False: a) In extractions, small proteins usually end up in the heavy phase. b) In extractions, large proteins usually end up in the heavy phase. c) Tie line length on a phase diagram has no effect on protein partitioning. d) For extraction, we assume that each stage reaches equilibrium. e) Isotherms that are concave down lead to self-sharpening solute fronts in adsorption. f) When scaling up an adsorption unit, the LUB scales proportionally with adsorber length. g) For chromatography, separation efficiency increases when HETP increases. h) In size exclusion chromatography, smaller molecules elute from the column first. i) In hydrophobic interaction chromatography, very hydrophobic proteins elute in water. j) Molecules at very high concentrations elute from the column as a perfect Gaussian peak. k) In chromatography scale-up, resolution will increase as column diameter increases. 1) A good adsorbent typically has no porosity. m) Polymer-based resins are usually more compressible than silica-based resins. n) Changing pH in an ion exchange chromatography process can alter protein binding. 으 o) Histidine residues will bind to immobilized Ni²+. 1

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
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1) Indicate whether each statement below is True or False: a) In extractions, small proteins usually end up in the heavy phase. b) In extractions, large proteins usually end up in the heavy phase. c) Tie line length on a phase diagram has no effect on protein partitioning. d) For extraction, we assume that each stage reaches equilibrium. e) Isotherms that are concave down lead to self-sharpening solute fronts in adsorption. f) When scaling up an adsorption unit, the LUB scales proportionally with adsorber length. g) For chromatography, separation efficiency increases when HETP increases. h) In size exclusion chromatography, smaller molecules elute from the column first. i) In hydrophobic interaction chromatography, very hydrophobic proteins elute in water. j) Molecules at very high concentrations elute from the column as a perfect Gaussian peak. k) In chromatography scale-up, resolution will increase as column diameter increases. 1) A good adsorbent typically has no porosity. m) Polymer-based resins are usually more compressible than silica-based resins. n) Changing pH in an ion exchange chromatography process can alter protein binding. 으 o) Histidine residues will bind to immobilized Ni²+. 1
Transcribed Image Text:1) Indicate whether each statement below is True or False: a) In extractions, small proteins usually end up in the heavy phase. b) In extractions, large proteins usually end up in the heavy phase. c) Tie line length on a phase diagram has no effect on protein partitioning. d) For extraction, we assume that each stage reaches equilibrium. e) Isotherms that are concave down lead to self-sharpening solute fronts in adsorption. f) When scaling up an adsorption unit, the LUB scales proportionally with adsorber length. g) For chromatography, separation efficiency increases when HETP increases. h) In size exclusion chromatography, smaller molecules elute from the column first. i) In hydrophobic interaction chromatography, very hydrophobic proteins elute in water. j) Molecules at very high concentrations elute from the column as a perfect Gaussian peak. k) In chromatography scale-up, resolution will increase as column diameter increases. 1) A good adsorbent typically has no porosity. m) Polymer-based resins are usually more compressible than silica-based resins. n) Changing pH in an ion exchange chromatography process can alter protein binding. 으 o) Histidine residues will bind to immobilized Ni²+. 1
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