17. Sucrose is hydrolyzed by the catalytic action of the enzyme sucrose as follows: sucrase sucrose Starting with a sucrose concentration → products C = S C = E 0 0.01 mM 0 1.0 mM and an enzyme concentration , the following kinetic data are obtained in a batch reactor: t (hr) 1 2 3 4 5 6 7 8 (mM) 9 C S 10 11 0.84 0.68 0.53 0.38 0.27 0.16 0.09 0.04 0.018 0.006 0.0025 Draw the profile of sucrose concentration across time. Determine the kinetic parameters of the enzyme using the linearization integral method. The correctness of the fitting to experimental data should be confirmed by calculating Determination Coefficient (R2), Root Mean Square Errors (RMSE) and Sum of Squared Errors (SSE). Give a graphical interpretation of the method used.
17. Sucrose is hydrolyzed by the catalytic action of the enzyme sucrose as follows: sucrase sucrose Starting with a sucrose concentration → products C = S C = E 0 0.01 mM 0 1.0 mM and an enzyme concentration , the following kinetic data are obtained in a batch reactor: t (hr) 1 2 3 4 5 6 7 8 (mM) 9 C S 10 11 0.84 0.68 0.53 0.38 0.27 0.16 0.09 0.04 0.018 0.006 0.0025 Draw the profile of sucrose concentration across time. Determine the kinetic parameters of the enzyme using the linearization integral method. The correctness of the fitting to experimental data should be confirmed by calculating Determination Coefficient (R2), Root Mean Square Errors (RMSE) and Sum of Squared Errors (SSE). Give a graphical interpretation of the method used.
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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17. Sucrose is hydrolyzed by the catalytic action of the enzyme sucrose as follows: sucrase sucrose Starting with a sucrose concentration → products C = S C = E 0 0.01 mM 0 1.0 mM and an enzyme concentration , the following kinetic data are obtained in a batch reactor: t (hr) 1 2 3 4 5 6 7 8 (mM) 9 C S 10 11 0.84 0.68 0.53 0.38 0.27 0.16 0.09 0.04 0.018 0.006 0.0025 Draw the profile of sucrose concentration across time. Determine the kinetic parameters of the enzyme using the linearization integral method. The correctness of the fitting to experimental data should be confirmed by calculating Determination Coefficient (R2), Root Mean Square Errors (RMSE) and Sum of Squared Errors (SSE). Give a graphical interpretation of the method used.
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