Microbiology with Diseases by Body System (4th Edition)
4th Edition
ISBN: 9780321918550
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Textbook Question
Chapter 4, Problem 1CM
Using the following terms, fill in the following concept map that describes
Decolorizer
Ethanol and acetone
Gram-negative bacteria
Gram-positive bacteria
Iodine
Primary stain
Purple
Safranin
Thin peptidoglycan layer
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describe the shape of the bacterial cells for each of the two yogurt bacteria. Be sure to use the terms for bacteria shapes that you learned in the Survey of the Microbial World lab!
Search for the following bacteria and complete the table below.
Bacterium
Stain
Description or sketch of cells with the specified feature
Flavobacterium capsulatum
Capsule stain
Corynebacterium diphtheriae
Methylene blue (metachromatic granules)
Mycobacterium tuberculosis
Acid fast stain
You must find up your OWN dichotomous key for all the possible unknown organisms listed below. The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures .
Possible Organisms
Alcaligenes faecalis
Enterobacter aerogenes
Enterococcus faecalis
Escherichia coli
Proteus vulgaris
Pseudomonas aeruginosa
Salmonella arizoniae
Staphylococcus aureus
Staphylococcus epidermidis
Staphylococcus saprophyticus
Streptococcusbovis
Streptococcus pyogenes
Note : The test must be performed using any of this test Mannitol Salt Agar, DNAse test, Catalase test, coagulase test, Novobiocin Test, BILE ESCULIN AGAR (BEA) TEST, Oxidase test, Bacitracin Sensitivity, Blood Agar . Macconkey, Nitrate Reduction test.
Thank you so much in Advance !
Chapter 4 Solutions
Microbiology with Diseases by Body System (4th Edition)
Ch. 4 - Prob. 1TMWCh. 4 - Why is a Gram-negative bacterium colorless but a...Ch. 4 - Why didnt Linnaeus create taxonomic groups for...Ch. 4 - Necrotizing Fasciitis Fever, chills, nausea,...Ch. 4 - Why is magnification high and color absent in an...Ch. 4 - Prob. 1MCCh. 4 - Prob. 2MCCh. 4 - Prob. 3MCCh. 4 - Curved glass lenses _________ light. a. refract b....Ch. 4 - Prob. 5MC
Ch. 4 - Prob. 6MCCh. 4 - Prob. 7MCCh. 4 - Prob. 8MCCh. 4 - Prob. 9MCCh. 4 - In the binomial system at nomenclature, which term...Ch. 4 - Prob. 1FIBCh. 4 - Prob. 2FIBCh. 4 - Prob. 3FIBCh. 4 - Fill in the Blanks 4. ___________ refers to...Ch. 4 - Fill in the Blanks 5. Cationic chromophores such...Ch. 4 - Prob. 1VICh. 4 - Label the microscope.Ch. 4 - Prob. 1SACh. 4 - Critique the following definition of magnification...Ch. 4 - Prob. 3SACh. 4 - Put the following substances in the order they are...Ch. 4 - Prob. 5SACh. 4 - Prob. 6SACh. 4 - Prob. 7SACh. 4 - Prob. 8SACh. 4 - Miki came home from microbiology lab with green...Ch. 4 - Why is the definition of species as successfully...Ch. 4 - With the exception of the discovery of new...Ch. 4 - Prob. 4CTCh. 4 - Prob. 5CTCh. 4 - In what ways are the Gram stain and the acid-fast...Ch. 4 - Prob. 7CTCh. 4 - Prob. 8CTCh. 4 - Why is the genus name Coccus placed within...Ch. 4 - A clinician obtains a specimen of urine from a...Ch. 4 - Using the following terms, fill in the following...
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- You must write up your OWN dichotomous key for all the possible unknown organisms listed below . The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures . Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes Imp Note - the test should be performed using any of the following tests stated below. Bile Escalin test, Oxidaze test, Blood Agar , Catalese Test, Mannitol salt agar . Thank you !arrow_forwardizzes/00071/take/questions/600381 Regarding Gram staining, what will be the appearance of the Gram-positive bacteria if... All steps are done correctly? The slide is not heat-fixed prior to staining? Crystal violet was omitted? lodine was omitted? Only iodine was used? Acetone:alcohol was omitted? Too much acetone:alcohol was used or was left on for too long? I Not enough acetone:alcohol was used? Carbol fuschin or safranin was omitted? W [Choose ] [Choose ] Purple Clear Pink Orange [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] 88 22°Carrow_forwardGram negative bacteria do not retain the stain when washed with alcohol.Comment.arrow_forward
- Based on the photo, describe the bacteria observed. - Gram Stain - Acid Fast Stainarrow_forwardOut of twelve tests that can be performed: Gram Stain Wet Mount Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test. Which four tests are the best fit to help identify bacteria that is unknown? Possible bacterias that are being observed are: Enterobacter aerogenes Escherichia coli Micrococcus luteus Proteus vulgaris Pseudomonas aeruginosa Salmonella typhimurium Serratia marcescens Staphylococcus aureus Staphylococcus epidermidisarrow_forwardCreate a dichotomous key Use the following bacteria and place them at the top of your key: Escherichia coli Klebsiella pneumoniae Staphylococcus epidermidis Bacillus subtilis Use the following five criteria to create the couplets: Morphology Gram reaction Acid Fast Reaction Endospore Positive/Negative Capsule Positive/Negativearrow_forward
- Complete the table below. Specify the simple stain used, the cell morphology, and a fun fact about the organism.arrow_forwardDescribe the bacteria observed in different staining procedures. Please refer to the photo - Gram Stain - Acid fast stainarrow_forwardComplete the missing results (dark grey cells in table) for the bacterial species. Solve for: Mycobacterium phlei Pseudomonas aeruginosa Staphylococcus aureusarrow_forward
- S aureus Styphimurium S aureus S typhimurium Nutrient Agar Plates MacConkey Agar Plates S epidermidis Unknown S epidermidis Unknown S aureus S typhimurium S aureus S typhimurium Mannitol Salt Agar Plates Gram Stain S epidermidis Unknown S epidermidis Unknownarrow_forwardOut of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and Staphylococcus epidermidis). Which of the following test(s) will help me identify the unknown gram-positive bacteria? Gram Stain Wet Mount (motility testing) Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Testarrow_forwardBased on the photo, describe the bacteria observed from the different staining technique. - Endospore Stain - Capsule Stainarrow_forward
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