Concept explainers
(a)
Interpretation:
The type of the radioactive material needs to be determined that is added to the culture medium considering that DNA is supposed to be radioactively labeled but not RNA, in dividing and growing bacterial cell.
Concept introduction:
Nucleotides are the construction buildings of
(b)
Interpretation:
A product comprising DNA polymerase and primed model DNA must be supplemented with precursors. Also, where core phosphorous atoms are evenly marked with 32P, the location of radioactive atoms in these precursors when the DNA is to be ready should be stated.
Concept introduction:
DNA elongation performs in 5’-3’direction. Chain elongation reaction takes place when 3-hydroxyl group attacks the innermost phosphorus atom of deoxy nucleoside triphosphate. In the
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Chapter 4 Solutions
BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
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- Please state and describe the four different types of non-covalent interactions.arrow_forwardPls help with these three questionsarrow_forward11. Which of the compounds below is the major product of the following reaction sequence? NOTE: PCC is pyridinium chlorochromate 1. BH 3 PCC 2. H2O2, NaOH NH HN ΗΝ, A B C CH3NH2, NaBH3CN D E NHarrow_forward
- 10. Which of the compounds below is the major organic product obtained from the following reaction sequence? Ph Ph Ph A B OH 1. EtMgBr H2CrO4 Zn(Hg), aq. HCI PhCHO ? 2. H₂O, H+ Ph. C D Ph "ར HO OH Earrow_forward7. What is the major organic product obtained from the following reaction sequence? Ph A OH 99 Ph OH D Br HOCH2CH2OH H2SO4 1. Mg, Et₂O 2. PhCH2CHO HCI, H₂O Br OH Ph Ph OH B C Br OH Ph Earrow_forwardPls helparrow_forward
- H₂N NH peptide_0e60 A dipeptide is made up of two (2) amino acids. The figure above shows one such dipeptide with an unknown sequence. Your task is to find out the two (2) letter sequence of this dipeptide.arrow_forwardcarbons in each of the structures below. For instance, the central carbon of chloromethylbutane (pictured 3. A chiral carbon is a carbon that is single-bonded to four different types of groups. Identify the chiral above) is a chiral carbon. (Can you see how the groups attached to it are all chemically different?) In each of the chiral molecules below, identify all the carbons that are chiral carbons by drawing a circle around each one of them. (a) the carbohydrate glucose H O (b) the carbohydrate fructose CH₂OH 1C H-C-OH 3 HO-C-H 4 H-C-OH 5 H-C-OH 6CH₂OH D-Glucose (linear form) (c) the amino acid leucine O O H3C. HO H H- -OH CH 3 NH2 H- -OH CH₂OH OHarrow_forwardWe always include controls in the Annexin-V-GFP/Propidium Iodide flow cytometric assay to study apoptosis. List four types of controls in this assay. Why do we need these controls? Explain your answers. After the flow assay, if we like to examine the morphology of the viable, early apoptotic and late apoptotic cells by confocal microscopy, what can we do and what are the expected results?arrow_forward
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