A reasonable explanation for the relation between % of GC base pairs in DNA and the melting temperature needs to be given. Concept introduction: The GC base pair i.e. Guanine - Cytosine pair help the DNA to maintain its helical structure which is dependent on the nucleotide sequences. A base pair is a unit structure which contains two nucleotides connected by hydrogen bonds to each other. These nucleotide strand connected by the hydrogen bond of the base pairs are stable at room temperature, but they separate above a melting point which is defined by the length of the molecule, the extent of mispairing and the amount of GC content in it. Higher GC content makes the melting point higher.

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Chapter 4, Problem 49P

Interpretation Introduction

Interpretation:

A reasonable explanation for the relation between % of GC base pairs in DNA and the melting temperature needs to be given.

Concept introduction:

The GC base pair i.e. Guanine - Cytosine pair help the DNA to maintain its helical structure which is dependent on the nucleotide sequences. A base pair is a unit structure which contains two nucleotides connected by hydrogen bonds to each other. These nucleotide strand connected by the hydrogen bond of the base pairs are stable at room temperature, but they separate above a melting point which is defined by the length of the molecule, the extent of mispairing and the amount of GC content in it. Higher GC content makes the melting point higher.

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