Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780321962751
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece
Publisher: PEARSON
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Chapter 39, Problem 4TYU
The binding of calcium to the troponin complex
- A. disrupts cross-bridges, allowing filaments to slide past each other.
- B. allows tropomyosin to bind actin.
- C. opens ion channels, allowing sodium to rush into the muscle cells.
- D. causes tropomyosin to shift position, exposing myosin bind sites on actin.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 39 Solutions
Campbell Biology in Focus (2nd Edition)
Ch. 39.1 - Contrast the role of Ca2+ in the contraction of a...Ch. 39.1 - WHAT IF? Why are the muscles of an animal that has...Ch. 39.1 - Prob. 3CCCh. 39.2 - Prob. 1CCCh. 39.2 - Prob. 2CCCh. 39.2 - Prob. 3CCCh. 39.3 - Prob. 1CCCh. 39.3 - MAKE CONNECTIONS How is the lunar-linked rhythm of...Ch. 39.4 - How might associative learning explain why...Ch. 39.4 - Prob. 2CC
Ch. 39.4 - MAKE CONNECTIONS How might a learned behavior...Ch. 39.5 - Prob. 1CCCh. 39.5 - MAKE CONNECTIONS Balancing selection can maintain...Ch. 39.6 - Prob. 1CCCh. 39.6 - Prob. 2CCCh. 39 - During the contraction of a vertebrate skeletal...Ch. 39 - Prob. 2TYUCh. 39 - According to Hamiltons rule, A. natural selection...Ch. 39 - The binding of calcium to the troponin complex A....Ch. 39 - Curare, a substance that blocks the acetylcholine...Ch. 39 - Prob. 6TYUCh. 39 - Prob. 7TYUCh. 39 - SCIENTIFIC INQUIRY Propose a hypothesis to explain...Ch. 39 - SCIENTIFIC INQUIRY Scientists studying scrub jays...Ch. 39 - Prob. 10TYUCh. 39 - FOCUS ON INFORMATION Learning is defined as a...Ch. 39 - SYNTHESIZE YOUR KNOWLEDGE Acorn woodpeckers...
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