Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 3.4, Problem 2MQ
Summary Introduction
A free energy is defined as the energy available to do work. It is abbreviated as “G”. It is released during the occurrence of
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 3 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 3.1 - Which four chemical elements make up the bulk of a...Ch. 3.1 - Which two classes of macromolecules contain most...Ch. 3.1 - Differentiate between trace metals and growth...Ch. 3.1 - Prob. 1CRCh. 3.2 - Compare and contrast simple transporters, the...Ch. 3.2 - Prob. 2MQCh. 3.2 - Cells of Escherichia coli transport lactose via...Ch. 3.3 - Prob. 1MQCh. 3.3 - Prob. 2MQCh. 3.3 - Prob. 1CR
Ch. 3.4 - What is free energy?Ch. 3.4 - Prob. 2MQCh. 3.4 - Using Table 3.2, calculate G0 for the reaction...Ch. 3.4 - Prob. 1CRCh. 3.5 - Prob. 1MQCh. 3.5 - Prob. 2MQCh. 3.5 - Prob. 3MQCh. 3.5 - Prob. 1CRCh. 3.6 - Prob. 1MQCh. 3.6 - Prob. 2MQCh. 3.6 - Prob. 3MQCh. 3.6 - Prob. 1CRCh. 3.7 - How much free energy is released when ATP is...Ch. 3.7 - Prob. 2MQCh. 3.7 - Prob. 3MQCh. 3.7 - Prob. 1CRCh. 3.8 - Which reactions in glycolysis are redox steps?Ch. 3.8 - Prob. 2MQCh. 3.8 - Prob. 3MQCh. 3.8 - How is ATP made in fermentation and in...Ch. 3.9 - How many molecules of CO2, NADH, and FADH2 are...Ch. 3.9 - What two major roles do the citric acid cycle and...Ch. 3.9 - Why is the glyoxylate cycle necessary for growth...Ch. 3.9 - Prob. 1CRCh. 3.10 - Prob. 1MQCh. 3.10 - Which electron carriers described in this section...Ch. 3.10 - List some of the key electron carriers found in...Ch. 3.11 - How do electron transport reactions generate the...Ch. 3.11 - How much energy is released per NADH oxidized...Ch. 3.11 - What structure in the cell links the proton motive...Ch. 3.11 - Prob. 1CRCh. 3.12 - Prob. 1MQCh. 3.12 - Prob. 2MQCh. 3.12 - Prob. 3MQCh. 3.12 - What is the major difference between aerobic...Ch. 3.13 - What form of activated glucose is used in the...Ch. 3.13 - Prob. 2MQCh. 3.13 - What functions does the pentose phosphate pathway...Ch. 3.13 - What is the importance of the enzyme...Ch. 3.14 - Prob. 1MQCh. 3.14 - List the steps required for the cell to...Ch. 3.14 - Which nitrogen bases are purines and which are...Ch. 3.14 - Prob. 1CRCh. 3.15 - Prob. 1MQCh. 3.15 - Prob. 2MQCh. 3.15 - Describe the process by which a fatty acid such as...Ch. 3 - Using the data of Figure 3.10, predict the...Ch. 3 - Prob. 2AQ
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