Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Textbook Question
Chapter 3.11, Problem 2MQ
- How much energy is released per NADH oxidized through the electron transport chain of Paracoccus shown in Figure 3 22? At which sites in the chain is the proton motive force being established?
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What is reverse electron transport and why is itnecessary in purple sulfur bacteria?
A denitrifying bacterium is strictly anaerobic. However, it possesses an electron
transport chain allowing « respiration ». The final acceptor is nitrate (NO3) that is
reduced in nitrite (NO₂). The electrons are introduced in the chain by the reduced
nicotinamide adenine dinucleotide coenzyme (NADH + H*).
1- Write the net reaction of the electron transport chain used by the bacterium.
2- Calculate the corresponding standard Gibbs (free) energy of reaction.
3- Is the anaerobic respiration (using nitrate) more or less exergonic than the aerobic
respiration (using O₂)? Justify your answer with numerical values.
When pure reduced cytochrome c is added to carefully prepared
mitochondria along with ADP, P, antimycin A, and oxygen, the
cytochrome c becomes oxidized, and ATP is formed, with a P/o
ratio approaching 1.0.
(a) Indicate the probable flow of electrons in this system.
(b) Why was antimycin A added?
(c) What does this experiment tell you about the location of cou-
pling sites for oxidative phosphorylation?
(d) Write a balanced equation for the overall reaction (including cyt
c oxidation and ATP synthesis).
Chapter 3 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 3.1 - Which four chemical elements make up the bulk of a...Ch. 3.1 - Which two classes of macromolecules contain most...Ch. 3.1 - Differentiate between trace metals and growth...Ch. 3.1 - Prob. 1CRCh. 3.2 - Compare and contrast simple transporters, the...Ch. 3.2 - Prob. 2MQCh. 3.2 - Cells of Escherichia coli transport lactose via...Ch. 3.3 - Prob. 1MQCh. 3.3 - Prob. 2MQCh. 3.3 - Prob. 1CR
Ch. 3.4 - What is free energy?Ch. 3.4 - Prob. 2MQCh. 3.4 - Using Table 3.2, calculate G0 for the reaction...Ch. 3.4 - Prob. 1CRCh. 3.5 - Prob. 1MQCh. 3.5 - Prob. 2MQCh. 3.5 - Prob. 3MQCh. 3.5 - Prob. 1CRCh. 3.6 - Prob. 1MQCh. 3.6 - Prob. 2MQCh. 3.6 - Prob. 3MQCh. 3.6 - Prob. 1CRCh. 3.7 - How much free energy is released when ATP is...Ch. 3.7 - Prob. 2MQCh. 3.7 - Prob. 3MQCh. 3.7 - Prob. 1CRCh. 3.8 - Which reactions in glycolysis are redox steps?Ch. 3.8 - Prob. 2MQCh. 3.8 - Prob. 3MQCh. 3.8 - How is ATP made in fermentation and in...Ch. 3.9 - How many molecules of CO2, NADH, and FADH2 are...Ch. 3.9 - What two major roles do the citric acid cycle and...Ch. 3.9 - Why is the glyoxylate cycle necessary for growth...Ch. 3.9 - Prob. 1CRCh. 3.10 - Prob. 1MQCh. 3.10 - Which electron carriers described in this section...Ch. 3.10 - List some of the key electron carriers found in...Ch. 3.11 - How do electron transport reactions generate the...Ch. 3.11 - How much energy is released per NADH oxidized...Ch. 3.11 - What structure in the cell links the proton motive...Ch. 3.11 - Prob. 1CRCh. 3.12 - Prob. 1MQCh. 3.12 - Prob. 2MQCh. 3.12 - Prob. 3MQCh. 3.12 - What is the major difference between aerobic...Ch. 3.13 - What form of activated glucose is used in the...Ch. 3.13 - Prob. 2MQCh. 3.13 - What functions does the pentose phosphate pathway...Ch. 3.13 - What is the importance of the enzyme...Ch. 3.14 - Prob. 1MQCh. 3.14 - List the steps required for the cell to...Ch. 3.14 - Which nitrogen bases are purines and which are...Ch. 3.14 - Prob. 1CRCh. 3.15 - Prob. 1MQCh. 3.15 - Prob. 2MQCh. 3.15 - Describe the process by which a fatty acid such as...Ch. 3 - Using the data of Figure 3.10, predict the...Ch. 3 - Prob. 2AQ
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- The photosynthetic process used by the green sulfur bacteria, under anaerobic conditions, is best represented by which of the following balanced equations? cell bio 6CO2 + 12H2S C6H12O6 + 6S2 + 6H2O C6H12O6 + C6H12O6 C12H22O11 + H2O 6CO2 + 12H2O C6H12O6 + 6O2 + 6H2O C12H22O11 + H2O C6H12O6 + C6H12O6 C6H12O6 + 6O2 + 6H2O 6CO2 + 12H2Oarrow_forwardIn several species of bacteria, GAPDH activity is controlled by the NADH/NAD+ ratio. Does the activity of GAPDH increase or decrease when the NADH/NAD+ ratio increases? Explain. Assume that only the forward direction of the reaction is relevant. a) The activity of GAPDH increases when the NADH/NAD+ ratio increases. b) The activity of GAPDH decreases when the NADH/NAD+ ratio increases.arrow_forwardThe photosynthetic process used by the green sulfur bacteria, under anaerobic conditions, is best represented by which of the following balanced equations? 6CO2 + 12H2O C6H12O6 + 6O2 + 6H2O 6CO2 + 12H2S C6H12O6 + 6S2 + 6H2O C12H22O11 + H2O C6H12O6 + C6H12O6 C6H12O6 + C6H12O6 C12H22O11 + H2O C6H12O6 + 6O2 + 6H2O 6CO2 + 12H2Oarrow_forward
- Define the term substrate-level phosphorylation:How does it differ from oxidative phosphorylation?What compound(s) do fermentative bacteria need tosynthesize in order to make ATP by substrate-levelphosphorylation?arrow_forwardYou experiementally apply the compund antimyctin, which inhinits the functioning of complex III, cytochrome reductase, from participating in redox reactions. You follow this with the addition of 6 molecules of FADH2. How many total ATP molecules can be created using the number of protons that were pumped?arrow_forwardDescribe the process of the electron transport chain. Include the role of the poton motive force, electron carriers, terminal electron acceptor. Where is the ETC located in euakryotes? where is it located in some prokaryotes? Do all organisms possess ETC?arrow_forward
- Bacteria growing under anaerobic conditions can respire using alternate electron donors and acceptors. One species has an electron transport chains with the following overall reactions. H2 + fumarate succinate. HCO2 + fumarate + 2H+→ HCO3 + succinate + H2O. Reaction Eº' Fumarate + 2H+ + 2e- → succinate 0.031V 2H+ + 2e- H2 -0.42 V HCO3 + 2e- + 2H+→ HCO2 + H2O -0.43 V a) Calculate how many ATP can be produced per electron for these electron transport systems? b) Can ubiquinone serve as the electron carrier in this system? look at reduction potentials c) Use your favourite search mechanism to find an electron carrier used. Please provide the reference.arrow_forwardFor Escherichia coli, why is more energy released in aerobicrespiration than during NO3- reduction?arrow_forwardWhat does electron transport in anammox bacteria have incommon with electron transport in purple sulfur bacteria?arrow_forward
- A chemolithoautotroph is respiring using only iron (Fe3+) and nitrite (NO2-). This bacterium does not consume organic molecules. How does this organism make NADH?arrow_forwardWhat is the order of the electron carriers in this alternative ETC?arrow_forwardInhibitors of electron transport have been used to determine the order of electron carriers. What would be the expected redox states of cytochrome a, bi, and c when the following inhibitors are added to succinate-driven respiring mitochondria? (i) myxothiazol (ii) antimycin A (iii) rotenonearrow_forward
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