Biology (MindTap Course List)
10th Edition
ISBN: 9781285423586
Author: Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher: Cengage Learning
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Chapter 34, Problem 14TYU
Summary Introduction
To draw: A simple cross section of a leaf and label the upper epidermis, lower epidermis, guard cells, spongy mesophyll, palisade mesophyll, and vascular tissue.
Concept introduction: Plants are non-motile living beings that are capable of producing their own food by utilizing the sunlight and carbon dioxide. They form the kingdom Plantae that includes all the plants such as trees, shrubs,
Summary Introduction
To determine: The cells that contain chloroplast.
Concept introduction:
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 34 Solutions
Biology (MindTap Course List)
Ch. 34.1 - Prob. 1LOCh. 34.1 - Prob. 2LOCh. 34.1 - Prob. 3LOCh. 34.1 - Prob. 4LOCh. 34.1 - How are leaves adapted to conserve water?Ch. 34.1 - Prob. 2CCh. 34.1 - What are the two types of vascular tissue in a...Ch. 34.1 - How is the leaf organized to deliver the raw...Ch. 34.2 - Prob. 5LOCh. 34.2 - Prob. 6LO
Ch. 34.2 - How does blue light trigger stomatal opening?Ch. 34.2 - Prob. 2CCh. 34.3 - Discuss transpiration and its effects on plants.Ch. 34.3 - Prob. 8LOCh. 34.3 - Prob. 1CCh. 34.3 - How do environmental factors (sunlight,...Ch. 34.3 - Prob. 3CCh. 34.3 - Prob. 4CCh. 34.4 - Define leaf abscission, explain why it occurs, and...Ch. 34.4 - Why do many woody plants living in temperate zones...Ch. 34.4 - Prob. 2CCh. 34.4 - Prob. 3CCh. 34.5 - Prob. 10LOCh. 34.5 - Prob. 1CCh. 34.5 - Prob. 2CCh. 34.5 - What are some of the specialized features of the...Ch. 34 - Prob. 1TYUCh. 34 - Prob. 2TYUCh. 34 - Prob. 3TYUCh. 34 - Prob. 4TYUCh. 34 - Which of the following is not an adaptation of...Ch. 34 - Prob. 6TYUCh. 34 - At sunrise, the accumulation in the guard cells of...Ch. 34 - Prob. 8TYUCh. 34 - Prob. 9TYUCh. 34 - Modified leaves that enable a stem to climb are...Ch. 34 - There is a trade-off between photosynthesis and...Ch. 34 - Suppose that you are asked to observe a micrograph...Ch. 34 - Prob. 13TYUCh. 34 - Prob. 14TYUCh. 34 - What might be some of the advantages of a plant...Ch. 34 - Prob. 16TYUCh. 34 - Prob. 17TYU
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