Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 32, Problem 5CHI
The fruit fly Drosophila melanogaster is an excellent model system. D. melanogaster hosts the ubiquitous insect symbiont Wolbachia pipientis, but unlike most other insect hosts, it is not subject to reproductive manipulation by the bacterium. Surprisingly, infection with W. pipientis protects the fruit fly from infection by three unrelated viruses that otherwise cause 100% mortality. Discuss the differences in this rickettsial-insect symbiosis with that described in Microbial Diversity & Ecology 32.1. Do you think it is likely that W. pipientis also protects wasps against other pathogens, such as viruses? Explain your answer from the point of view of the bacterium and from the insect’s point of view.
Read the original paper: Hedges, L. M. 2008. Wolbachia and virus protection in insects. Science 322:722.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 32 Solutions
Prescott's Microbiology
Ch. 32.1 - What methanogenic substrates are also available in...Ch. 32.1 - What is the nutritional type of the endosymbiotic...Ch. 32.1 - Prob. 3MICh. 32.1 - How does myxobacterial predation differ from that...Ch. 32.1 - How could one test to see if an insect-microbe...Ch. 32.1 - What is the role of the Riftia tube worms...Ch. 32.1 - Prob. 1.3RIACh. 32.1 - What is meant by tight trophic coupling between...Ch. 32.1 - Why is it important that the rumen is a reducing...Ch. 32.1 - Describe the mutualism between methanogenic...
Ch. 32.1 - How does cooperation differ from mutualism? What...Ch. 32.1 - Why is the X. nematophilaS. carpocapsae symbiosis...Ch. 32.1 - Prob. 3.1RIACh. 32.1 - Why is nitrification a good example of a...Ch. 32.1 - Define predation and parasitism. How are these...Ch. 32.1 - Prob. 3.4RIACh. 32.1 - What is a lichen? Why is this considered an...Ch. 32.1 - Describe the amensalism observed in attine ant...Ch. 32.1 - Prob. 4.2RIACh. 32.1 - What is the competitive exclusion principle? List...Ch. 32.2 - How might a microbial symbiont affect the uptake...Ch. 32.2 - Cite three reasons a germfree mouse would be more...Ch. 32.2 - People who suffer from colitis often find that...Ch. 32.3 - 1. Why is it important to understand the normal...Ch. 32.3 - Why is the skin not always a favorable...Ch. 32.3 - How do microorganisms contribute to body odor?Ch. 32.3 - Prob. 1.4RIACh. 32.3 - Prob. 2.1RIACh. 32.3 - What physiological processes move the microbiota...Ch. 32.3 - What is the role of pH in determining the...Ch. 32.3 - How would you define an opportunistic...Ch. 32 - Describe an experimental approach to determine if...Ch. 32 - Prob. 2CHICh. 32 - Prob. 3CHICh. 32 - Compare and contrast the microbial communities...Ch. 32 - The fruit fly Drosophila melanogaster is an...
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