Biology: Life on Earth with Physiology (11th Edition)
11th Edition
ISBN: 9780133923001
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Chapter 31.1, Problem 2CYL
Summary Introduction
To explain:
The importance of three levels of biodiversity that conservation biologists study and seek to protect.
Introduction:
Biodiversity represents a habitat where the entire living organism like plants, animals, humans, and microorganism live together.
Three level of biodiversity is genetic biodiversity, species biodiversity, and ecosystem biodiversity.
The important role of these three levels of biodiversity is to maintain the balance of the ecosystem, the habitat of species, and food chain and food web.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 31 Solutions
Biology: Life on Earth with Physiology (11th Edition)
Ch. 31.1 - describe the goals of conversation biology?Ch. 31.1 - Prob. 2CYLCh. 31.2 - Prob. 1CSCCh. 31.2 - describe the major categories of ecosystem...Ch. 31.2 - Prob. 1TCCh. 31.2 - Prob. 2CYLCh. 31.3 - define mass extinction?Ch. 31.3 - Prob. 1HYEWCh. 31.3 - explain why biologists fear that a mass extinction...Ch. 31.4 - Prob. 1CSC
Ch. 31.4 - Prob. 1CYLCh. 31.4 - Prob. 1TCCh. 31.4 - Prob. 2CYLCh. 31.5 - Prob. 1CSCCh. 31.5 - Prob. 1CYLCh. 31.5 - Prob. 1TCCh. 31.5 - Prob. 2CYLCh. 31.6 - Before 1700, wolves roamed over almost all of...Ch. 31.6 - describe the principles of sustainable...Ch. 31.6 - In 1970, Atlantic leatherback sea turtle...Ch. 31.6 - explain how population, technology, and lifestyle...Ch. 31 - List some reasons that the ecological footprints...Ch. 31 - Prob. 1FIBCh. 31 - A factor that increases humanity ecological...Ch. 31 - Prob. 1RQCh. 31 - Search for and describe some examples of habitat...Ch. 31 - Prob. 2FIBCh. 31 - Prob. 2MCCh. 31 - What is ecological economics? Why is it important?Ch. 31 - Prob. 3FIBCh. 31 - Prob. 3MCCh. 31 - Prob. 3RQCh. 31 - Prob. 4FIBCh. 31 - Prob. 4MCCh. 31 - Prob. 4RQCh. 31 - The smallest population of a species that is...Ch. 31 - Which of the following is not true of a population...Ch. 31 - Why are efforts to protect monarch butterflies a...Ch. 31 - A Native American saying tells us that We do not...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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