SAPLINGPLUS F/BIOCHEM+ICLICKER REEF-CODE
SAPLINGPLUS F/BIOCHEM+ICLICKER REEF-CODE
9th Edition
ISBN: 9781319398583
Author: BERG
Publisher: MAC HIGHER
Question
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Chapter 31, Problem 12P
Interpretation Introduction

(a)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of 32P-labelled ATP formation from 32PPi is to be done by ATP and 32PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond. 

Translation is the mechanism via which mRNA is decoded and translated into a protein.

SAPLINGPLUS F/BIOCHEM+ICLICKER REEF-CODE, Chapter 31, Problem 12P , additional homework tip  1

Interpretation Introduction

(b)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of 32P-labelled ATP formation from 32PPi is to be done by ATP and 32PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond. 

Translation is the mechanism via which mRNA is decoded and translated into a protein.

SAPLINGPLUS F/BIOCHEM+ICLICKER REEF-CODE, Chapter 31, Problem 12P , additional homework tip  2

Interpretation Introduction

(c)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of 32P-labelled ATP formation from 32PPi is to be done by isoleucine, ATP and32PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds,and the triphosphate is attached to the ribose sugar by a phosphoester bond. 

Translation is the mechanism via which mRNA is decoded and translated into a protein.

SAPLINGPLUS F/BIOCHEM+ICLICKER REEF-CODE, Chapter 31, Problem 12P , additional homework tip  3

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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD.  The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?
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